Draft Study on the homology of the genomes of tetraploid Asiatic lilies ( Lilium ) using FISH ( Fluorescence in situ hybridization )

Asiatic lily cultivars, bred by hybridization and/or chromosome doubling of species of section Sinomartagon of Lilium, are diploid, triploid or tetraploid, but the homology of the genomes among Sinomartagon species and Asiatic lilies remains unclear. In the present research, two tetraploid Asiatic cultivars were analyzed, using 45S rDNA as probe, for their FISH karyotypes and their chromosomal association, anaphase I, telophase II and pollen viability were surveyed to assess the multivalent segregation. Chromosomal assortment of six progenies of the two tetraploid cultivars were also investigated. The results showed that the tetraploid cultivars had similar FISH karyotypes, they predominantly formed multivalents, and these were equally separated because their anaphase I, telophase II and their pollen viability are normal, as those of diploid species; apart from minor variations, FISH karyotypes of progenies were similar to each other and to their parents. Based on these results and considering the high crossability among Sinomartagon species and/ or Asiatic lilies, we concluded that Sinomartagon species and their resulting cultivars share a common genome, so polyploidy Asiatic lilies are autopolyploid. Page 2 of 27 https://mc06.manuscriptcentral.com/genome-pubs Genome

[1]  P. Arens,et al.  GISH analyzed progenies generated from allotriploid lilies as female parent , 2015 .

[2]  Shujun Zhou,et al.  Study on lily introgression breeding using allotriploids as maternal parents in interploid hybridizations , 2014, Breeding science.

[3]  Z. Shu.  Technical Ways of Breeding Modern Lily(Lilium) Cultivars and the Mechanisms of the Special Phenomena of Their Hybridizations , 2014 .

[4]  K. Li,et al.  ANALYSIS OF THE VARIATION OF 18S-5.8S-28S RDNA LOCI IN ASIATIC, ORIENTAL AND LONGIFLORUM LILIES (LILIUM) USING FLUORESCENCE IN SITU HYBRIDIZATION , 2012 .

[5]  Gui-xing Ren,et al.  Genomic variation of new cultivars selected from distant hybridization in Lilium , 2012 .

[6]  Kehu Li,et al.  Analysis of endosperm development of allotriploid × diploid/tetraploid crosses in Lilium , 2011, Euphytica.

[7]  P. Arens,et al.  Lilium: breeding history of the modern cultivar assortment , 2011 .

[8]  Guo Fang-qi.  Studies on Ploidy Levels,Pollen Fertility and Interploid Hybridization of Asiatic Lilies , 2011 .

[9]  Guo Fang-qi.  Observation on Ploidy Levels of Lily Cultivars , 2011 .

[10]  J. Bang,et al.  Physical Mapping of rRNA Gene Loci and Inter-specific Relationships in Wild Lilium Distributed in Korea , 2010, Journal of Plant Biology.

[11]  B. Jiang,et al.  Cytological studies on meiosis and male gametophyte development in autotetraploid cucumber , 2010, Biologia Plantarum.

[12]  Christian Parisod,et al.  Evolutionary consequences of autopolyploidy. , 2010, The New phytologist.

[13]  J. M. Tuyl,et al.  Simultaneous identification of three different genomes in Lilium hybrids through multicolour GISH. , 2010 .

[14]  Pamela S Soltis,et al.  The role of hybridization in plant speciation. , 2009, Annual review of plant biology.

[15]  Gaisheng Zhang,et al.  Meiotically asynapsis-induced aneuploidy in autopolyploid Arabidopsis thaliana , 2009, Journal of Plant Research.

[16]  R. Visser,et al.  Analysis of the meiosis in the F(1) hybrids of Longiflorum x Asiatic (LA) of lilies (Lilium) using genomic in situ hybridization. , 2008, Journal of genetics and genomics = Yi chuan xue bao.

[17]  R. Visser,et al.  Genome composition of triploid lily cultivars derived from sexual polyploidization of Longiflorum × Asiatic hybrids (Lilium) , 2008, Euphytica.

[18]  E. Jacobsen,et al.  Meiotic behaviour of individual chromosomes in allotriploid Alstroemeria hybrids , 2004, Heredity.

[19]  A. Marasek,et al.  Determination by GISH and FISH of hybrid status in Lilium. , 2004, Hereditas.

[20]  E. Jacobsen,et al.  Occurrence of numerically unreduced (2n) gametes in Alstroemeria interspecific hybrids and their significance for sexual polyploidisation , 2003, Euphytica.

[21]  G. Jones,et al.  Meiosis in autopolyploid Crepis capillaris , 1993, Chromosoma.

[22]  E. Jacobsen,et al.  Relevance of sexual polyploidization for crop improvement – A review , 2004, Euphytica.

[23]  R. Visser,et al.  Molecular characterization and physical localization of highly repetitive DNA sequences from Brazilian Alstroemeria species , 2004, Chromosome Research.

[24]  K. Lim,et al.  Introgression of Lilium Rubellum Baker Chromosomes into L. Longiflorum Thunb.: A Genome Painting Study of the F1 Hybrid, BC1 and BC2 Progenies , 2004, Chromosome Research.

[25]  E. Sanchez-Moran,et al.  Partial diploidization of meiosis in autotetraploid Arabidopsis thaliana. , 2003, Genetics.

[26]  J. Małuszyńska,et al.  Chromosomal rearrangement in autotetraploid plants of Arabidopsis thaliana. , 2004, Hereditas.

[27]  M. Nakano,et al.  Interspecific hybrids between Lilium nobilissimum and L. regale produced via ovules-with-placental-tissue culture , 2000 .

[28]  K. Lim,et al.  Breakthroughs in interspecific hybridization of lily , 2000 .

[29]  K. Lim,et al.  Homoeologous recombination in 2n-gametes producing interspecific hybrids of Lilium (Liliaceae) studied by genomic in situ hybridization (GISH) , 1999 .

[30]  E. Jacobsen,et al.  Homoeologous chromosome pairing in the distant hybrid Alstroemeria aurea × A. inodora and the genome composition of its backcross derivatives determined by fluorescence in situ hybridization with species-specific probes , 1999, Heredity.

[31]  E. Macrae.  Lilies: a guide for growers and collectors. , 1998 .

[32]  K. Okazaki,et al.  Interspecific hybrids between Lilium 'Oriental' hybrid and L. 'Asiatic' hybrid produced by embryo culture with revised media , 1994 .

[33]  R. Bino,et al.  Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses , 1991 .

[34]  W. Gerlach,et al.  Cloning and characterization of ribosomal RNA genes from wheat and barley. , 1979, Nucleic acids research.