The tobacco wounding-activated mitogen-activated protein kinase is encoded by SIPK.

It has been demonstrated that both salicylic acid and fungal elicitors activate a 48-kDa mitogen-activated protein kinase termed salicylic acid-induced protein kinase (SIPK) in tobacco suspension cells. Here, we show that infiltration of these agents into tobacco leaves also activates SIPK. Of particular interest, infiltration of water alone activated a kinase of the same size, possibly because of wounding and/or osmotic stresses. The kinetics of kinase activation, however, differ for these different treatments. Various mechanical stresses, including cutting and wounding by abrasion, also activated a 48-kDa kinase. By using an immune-complex kinase assay with antibodies specific for SIPK or wounding-induced protein kinase, we demonstrate that this wounding-activated 48-kDa kinase is SIPK, rather than wounding-induced protein kinase, as reported [Seo, S., Okamoto, M., Seto, H., Ishizuka, K., Sano, H. & Ohashi, Y. (1995) Science 270, 1988-1992]. Activation of SIPK after wounding was associated with tyrosine phosphorylation but not with increases in SIPK mRNA or protein levels. Thus, the same mitogen-activated protein kinase, SIPK, appears to facilitate signaling for two distinct pathways that lead to disease resistance responses and wounding responses.

[1]  D. Klessig,et al.  Activation of the Tobacco SIP Kinase by Both a Cell Wall–Derived Carbohydrate Elicitor and Purified Proteinaceous Elicitins from Phytophthora spp , 1998, Plant Cell.

[2]  M. E. Hoyos,et al.  Rapid and Transient Activation of a Myelin Basic Protein Kinase in Tobacco Leaves Treated with Harpin from Erwinia amylovora , 1997, Plant physiology.

[3]  J. Stratmann,et al.  Myelin basic protein kinase activity in tomato leaves is induced systemically by wounding and increases in response to systemin and oligosaccharide elicitors. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[4]  D. Klessig,et al.  Signal perception and transduction in plant defense responses. , 1997, Genes & development.

[5]  D Scheel,et al.  Receptor-mediated activation of a MAP kinase in pathogen defense of plants. , 1997, Science.

[6]  R. Dixon,et al.  THE OXIDATIVE BURST IN PLANT DISEASE RESISTANCE. , 1997, Annual review of plant physiology and plant molecular biology.

[7]  D. Klessig,et al.  Salicylic acid activates a 48-kD MAP kinase in tobacco. , 1997, The Plant cell.

[8]  C. Hwang,et al.  Sequences Necessary for Nitrate-Dependent Transcription of Arabidopsis Nitrate Reductase Genes , 1997, Plant physiology.

[9]  H. Hirt,et al.  Wounding Induces the Rapid and Transient Activation of a Specific MAP Kinase Pathway. , 1997, The Plant cell.

[10]  Heribert Hirt,et al.  Multiple roles of MAP kinases in plant signal transduction , 1997 .

[11]  K. Shinozaki,et al.  Environmental stress response in plants: the role of mitogen-activated protein kinases. , 1997, Trends in biotechnology.

[12]  G. Howe,et al.  Polypeptide signaling for plant defensive genes exhibits analogies to defense signaling in animals. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[13]  J. Avruch,et al.  Sounding the Alarm: Protein Kinase Cascades Activated by Stress and Inflammation* , 1996, The Journal of Biological Chemistry.

[14]  H. Hirt,et al.  Stress signaling in plants: a mitogen-activated protein kinase pathway is activated by cold and drought. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[15]  J. Avruch,et al.  Protein kinase cascades activated by stress and inflammatory cytokines , 1996, BioEssays : news and reviews in molecular, cellular and developmental biology.

[16]  Xiaozhong Wang,et al.  Stress-Induced Phosphorylation and Activation of the Transcription Factor CHOP (GADD153) by p38 MAP Kinase , 1996, Science.

[17]  S. Chandra,et al.  Activation of Phospholipase A by Plant Defense Elicitors , 1996, Plant physiology.

[18]  K. Irie,et al.  A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[19]  T. Tan,et al.  Persistent Activation of c-Jun N-terminal Kinase 1 (JNK1) in γ Radiation-induced Apoptosis (*) , 1996, The Journal of Biological Chemistry.

[20]  H. Sano,et al.  Tobacco MAP Kinase: A Possible Mediator in Wound Signal Transduction Pathways , 1995, Science.

[21]  S. Chandra,et al.  Evidence for a Mechanically Induced Oxidative Burst , 1995, Plant physiology.

[22]  Y. Ito,et al.  Cutting activates a 46-kilodalton protein kinase in plants. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[23]  S. Jin,et al.  Identification of a Plant-Encoded Analog of PKR, the Mammalian Double-Stranded RNA-Dependent Protein Kinase , 1995, Plant physiology.

[24]  E. Krebs,et al.  The MAPK signaling cascade , 1995, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[25]  M. Parniske,et al.  Oligopeptide elicitor-mediated defense gene activation in cultured parsley cells. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[26]  E. Weiler,et al.  Oligogalacturonides and chitosan activate plant defensive genes through the octadecanoid pathway. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[27]  H. Shinshi,et al.  Transient Activation and Tyrosine Phosphorylation of a Protein Kinase in Tobacco Cells Treated with a Fungal Elicitor. , 1995, The Plant cell.

[28]  I. Herskowitz.  MAP kinase pathways in yeast: For mating and more , 1995, Cell.

[29]  M. Mehdy.  Active Oxygen Species in Plant Defense against Pathogens , 1994, Plant physiology.

[30]  S. Zhang,et al.  Fungal Elicitor-Induced Bean Proline-Rich Protein mRNA Down-Regulation Is Due to Destabilization That Is Transcription and Translation Dependent. , 1993, The Plant cell.

[31]  H. Hirt,et al.  The plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner. , 1993, The Plant journal : for cell and molecular biology.

[32]  Roger J. Davis,et al.  cPLA2 is phosphorylated and activated by MAP kinase , 1993, Cell.

[33]  T. Jacobs,et al.  MsERK1: a mitogen-activated protein kinase from a flowering plant. , 1993, The Plant cell.

[34]  R. Dixon,et al.  Molecular Communication in Interactions Between Plants and Microbial Pathogens , 1990 .