[7] Structure and interactions of proteins in solution studied by small-angle neutron scattering

Publisher Summary The use of the small-angle neutron scattering (SANS) technique as an analytical tool for the study of global structures of macromolecules in solution follows naturally from the earlier development of the small-angle X-ray scattering (SAXS) technique. This chapter presents the method of SANS as applied to soluble globular proteins in solution. Its advantages over the SAXS technique are also brought out in the discussion. The emphasis is put on analysis of scattering data from systems at both low and moderately high concentrations, which better simulate physiological conditions. Owing to the considerable number of charges on proteins in solution at pH 7, there exists a long range electrostatic interaction between the molecules even at the 1% (g/dl) concentration level. It should be emphasized that the dilute regime where interactions can be neglected is an impractical regime in which to perform experiments because of the severe loss in scattered intensity for solutions with less than 0.1% protein. For globular proteins with known conformation, experiments performed at high concentrations provide additional information, such as the effective surface charge, the amount of bound water, and the local spatial ordering of the molecules in solution.

[1]  H. Stuhrmann,et al.  Small-angle scattering of biological structures , 1978 .

[2]  O. Kratky X-RAY SMALL ANGLE SCATTERING WITH SUBSTANCES OF BIOLOGICAL INTEREST IN DILUTED SOLUTIONS. , 1963, Progress in biophysics and molecular biology.

[3]  P. Debye Light Scattering in Solutions , 1944 .

[4]  D. Bendedouch,et al.  Study of intermicellar interaction and structure by small angle neutron scattering , 1983 .

[5]  K. Ibel Comparison of neutron and X-ray scattering of dilute myoglobin solutions. , 1975, Journal of molecular biology.

[6]  B. Schoenborn,et al.  An analysis by low-angle neutron scattering of the structure of the acetylcholine receptor from Torpedo californica in detergent solution. , 1979, Biophysical journal.

[7]  J. Hayter,et al.  An analytic structure factor for macroion solutions , 1981 .

[8]  John B. Hayter,et al.  A rescaled MSA structure factor for dilute charged colloidal dispersions , 1982 .

[9]  Sow-Hsin Chen,et al.  Determination of interparticle structure factors in ionic micellar solutions by small angle neutron scattering , 1983 .

[10]  G. Zaccai,et al.  Determination of molecular weight by neutron scattering , 1981 .

[11]  W. Schmatz,et al.  Multiple-scattering treatment for small-angle scattering problems , 1980 .

[12]  P. Laggner,et al.  Neutron small angle scattering on selectively deuterated human plasma low density lipoproteins. The location of polar phospholipid headgroups. , 1981, The Journal of biological chemistry.

[13]  D. Engelman,et al.  Determination of quaternary structure by small angle neutron scattering. , 1975, Annual review of biophysics and bioengineering.

[14]  B. Fedorov,et al.  Sperm whale myoglobin structure in solution differs from its structure in crystal by a shift of the ‘hairpin’ Gh , 1978, FEBS letters.

[15]  Sow-Hsin Chen,et al.  Effect of an attractive potential on the interparticle structure of ionic micelles at high salt concentration , 1984 .

[16]  B. Jacrot,et al.  REVIEW ARTICLE: The study of biological structures by neutron scattering from solution , 1976 .

[17]  Sow-Hsin Chen,et al.  Structure and interparticle interactions of bovine serum albumin in solution studied by small-angle neutron scattering , 1983 .