Validation of a mathematical model for determining the Yin-Yang nature of fruits.

A mathematical model for determining the Yin Yang nature of fruits was established in our previous study. The objective of this study was to validate the mathematical model using animal experiments. One hundred and twenty Wistar II female rats were randomly divided into five diet groups: A, B, C, D and E that were administered with saline solution, hot Traditional Chinese Medicine (TCM) tonic, cold TCM tonic, hot (Yang) formulated mineral solution and cold (Yin) formulated mineral solution, respectively. The consumption of drinking water of rats in each group was determined during a feeding period of 21 days. On the last day of the experiment, the lingual superficial structure of the rats was examined, which is a practice in traditional Chinese medicine to diagnose Yin-yang symptoms, and blood samples were collected from the rats to determine serum thrombocytin (5-HT) and thyrotrophic hormone (TSH) and plasma noradrenaline (NE). The diet D group, administered with the Yang mineral solutions demonstrated the same trend as the diet B group fed with hot TCM tonic (hot control), while the diet E group administered with the Yin mineral solutions has the similar trend as diet C group fed with cold TCM tonic (cold control). It was concluded that the diet D had Yang nature, whereas the diet E had Yin nature. The results from current study confirmed the findings from the previous study that the Yin-Yang nature of the fruits could be determined by the ratio of copper, iron and magnesium content using the mathematical model.