Changes in Digestive Enzyme Activities of northern sheatfish After feeding
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The effect of feeding on the activity of protease、amylase and lipase in the main digestive organs of northern sheatfish was studied.The results showed that the activity of protease in stomach decreased after feeding,then increased and kept at a high level after 3h of feeding,which was 126.40±18.96 U/mg protein and presented a striking contrast to feeding ago(P0.05).But the activity of protease in intestinal canal increased all along and kept at a higher level after 4h of feeding,which was 70.68±9.87 U/mg protein.The effect of feeding on the activity of amylase indicated that the amylase in stomach decreased after 1h of feeding(P0.05),then increased and kept at a higher level after 3h of feeding,which was 15.87±2.02 U/mg protein.Trend of amylase activities in stomach change appeared from low to high.The activity of amylase in intestinal canal kept at a higher level after 5h of feeding.At the same time,the results showed the activity of lipase in stomach changed quite acutely,which kept at a higher level after 3h of feeding,but the activity of lipase in intestinal canal changed slack,The feeding effect the activity of lipase in stomach was more distinct than the activity of lipase in intestinal canal.