Supplemental Data Resveratrol Delays Age-Related Deterioration and Mimics Transcriptional Aspects of Dietary Restriction without Extending Life Span

Animals and diets. Male C57BL/6NIA mice at 11 months of age were purchased from the National Institute on Aging Aged Rodent Colony (Harlan Sprague-Dawley, Indianapolis, IN), and maintained on a standard purified mouse diet (AIN-93G) for one month prior to the start of the experiment. Beginning at one year of age, SD and EOD groups were fed a standard AIN-93G diet or AIN-93G plus 0.01% or 0.04% resveratrol for the duration of the study. Three separate groups were placed on an HC diet (AIN-93G modified by the addition of hydrogenated coconut oil to provide 60% of calories from fat) or HC + 0.01% or 0.04% resveratrol 6 weeks later and remained on those diets throughout the study. SD and HC mice were fed ad libitum. EOD mice were fed ad libitum on alternate days then moved to a separate cage without food for 24 h (to prevent food from being stored in the bedding). Food was prepared and stored as described previously (Baur et al., 2006). The mice were maintained on a 12 hour light/dark cycle and between 68-72°F according to animal protocols and NIH guidelines. Food intake and body weight were measured on a weekly basis for the duration of the study. Both 40% restriction and EOD are well established means to improve health and extend lifespan in rodents. Both involve a component of food deprivation and show common effects such as increasing insulin sensitivity and blocking cancer growth. Our primary reason in