THz spectroscopy based high sensitivity measurement of protein using a metal mesh device

A label-free bioaffinity sensor working in terahertz (THz) region with a nitrocellulose membrane filter was demonstrated, which is based on the resonant transmission phenomenon and the dip in the spectra of the metal mesh device. By using this sensor, we succeeded in the highly sensitive detection of small amounts of protein avidin-biotin complex. A distinct change of transmittance caused by shift of the transmission dip was observed for 8 ng/mm2 (74 fmol) of horseradish peroxidase (HRP) labeled avidin. The sensing method has broad utility for many reactions on the membrane filter as a simple and rapid sensor.

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