Purification of recombinant proteins by fusion with thermally-responsive polypeptides
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[1] M. Dewhirst,et al. Enhanced delivery of a monoclonal antibody F(ab')2 fragment to subcutaneous human glioma xenografts using local hyperthermia. , 1990, Cancer research.
[2] John E. Coligan,et al. Current Protocols in Protein Science , 1996 .
[3] L. Vértesy,et al. Tendamistat (HOE 467), a tight-binding α-amylase inhibitor from Streptomyces tendae 4158 , 1984 .
[4] T. M. Parker,et al. Temperature of polypeptide inverse temperature transition depends on mean residue hydrophobicity , 1991 .
[5] H. Michel,et al. A versatile plasmid expression vector for the production of biotinylated proteins by site-specific, enzymatic modification in Escherichia coli. , 1996, Gene.
[6] D. Urry,et al. Phase‐structure transitions of the elastin polypentapeptide–water system within the framework of composition–temperature studies , 1985, Biopolymers.
[7] B. Slatko,et al. An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. , 1988, Gene.
[8] Allan S. Hoffman,et al. Applications of thermally reversible polymers and hydrogels in therapeutics and diagnostics , 1987 .
[9] Jie Xu,et al. Product purification by reversible phase transition following Escherichia coli expression of genes encoding up to 251 repeats of the elastomeric pentapeptide GVGVP. , 1996, Protein expression and purification.
[10] D. Smith,et al. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. , 1988, Gene.
[11] Dan W. Urry,et al. Entropic elastic processes in protein mechanisms. I. Elastic structure due to an inverse temperature transition and elasticity due to internal chain dynamics , 1988, Journal of protein chemistry.
[12] J. Porath. Immobilized metal ion affinity chromatography. , 1992, Protein expression and purification.
[13] Mathias Uhlén,et al. Fusion proteins in biotechnology and structural biology , 1992, Current Biology.
[14] H. Hsiung,et al. Chelating peptide-immobilized metal ion affinity chromatography , 1989 .
[15] R. McGraw,et al. Production of recombinant porcine tumor necrosis factor alpha in a novel E. coli expression system. , 1992, BioTechniques.
[16] A S Hoffman,et al. Polymer-protein conjugates. II. Affinity precipitation separation of human immunogammaglobulin by a poly(N-isopropylacrylamide)-protein A conjugate. , 1990, Biomaterials.
[17] M. Dewhirst,et al. Local hyperthermia improves uptake of a chimeric monoclonal antibody in a subcutaneous xenograft model. , 1997, Clinical cancer research : an official journal of the American Association for Cancer Research.
[18] A. Holmgren,et al. Enzymatic reduction-oxidation of protein disulfides by thioredoxin. , 1984, Methods in enzymology.
[19] R. Raines,et al. Ribonuclease S‐peptide as a carrier in fusion proteins , 1993, Protein science : a publication of the Protein Society.
[20] M. Uhlén,et al. Gene fusions for purpose of expression: an introduction. , 1990, Methods in enzymology.
[21] D. Urry. Physical Chemistry of Biological Free Energy Transduction As Demonstrated by Elastic Protein-Based Polymers† , 1997 .
[22] C. Pidgeon,et al. Chelating peptide-immobilized metal ion affinity chromatography. A new concept in affinity chromatography for recombinant proteins. , 1988, The Journal of biological chemistry.
[23] J. Mccoy,et al. A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli. , 1998, Nucleic acids research.
[24] J. Mccoy,et al. A Thioredoxin Gene Fusion Expression System That Circumvents Inclusion Body Formation in the E. coli Cytoplasm , 1993, Bio/Technology.
[25] A S Hoffman,et al. Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. , 1994, Bioconjugate chemistry.
[26] D. Urry. Free energy transduction in polypeptides and proteins based on inverse temperature transitions. , 1992, Progress in biophysics and molecular biology.
[27] M. Uhlén,et al. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. , 1997, Protein expression and purification.
[28] J. Greenwood,et al. The cellulose-binding domains of cellulases: tools for biotechnology , 1989 .