Detection of canine adenovirus 1 in red foxes (Vulpes vulpes) and raccoons (Procyon lotor) in Germany with a TaqMan real-time PCR assay

We developed a real-time (rt)PCR assay based on TaqMan probe technology for the specific detection of canine adenovirus 1 (CAdV-1). The assay is able to detect three 50% tissue culture infectious dose/mL in CAdV-1–containing cell culture supernatant. Viral genomes were not amplified of canine adenovirus 2 or of several bovine, porcine, and avian adenoviruses. In silico analysis provided no indication of amplification of other heterologous genomes. The sensitivity of the real-time assay exceeded that of a conventional gel-based CAdV-1 PCR by a factor of 100. Following the integration of the novel PCR into the Hessian wildlife-monitoring program, CAdV-1 DNA was detected in none of the tested raccoons (n = 48) but in 11 of 97 foxes.

[1]  E. Dubovi,et al.  Patterns of Exposure of Iberian Wolves (Canis lupus) to Canine Viruses in Human-Dominated Landscapes , 2016, EcoHealth.

[2]  V. Martella,et al.  A duplex real-time PCR assay based on TaqMan technology for simultaneous detection and differentiation of canine adenovirus types 1 and 2. , 2016, Journal of virological methods.

[3]  F. Dondi,et al.  Development of a SYBR Green real-time PCR assay with melting curve analysis for simultaneous detection and differentiation of canine adenovirus type 1 and type 2. , 2015, Journal of virological methods.

[4]  J. Oem,et al.  CANINE ADENOVIRUS TYPE 1 IN A FENNEC FOX (VULPES ZERDA) , 2014, Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians.

[5]  F. Dondi,et al.  Investigation of the presence of canine adenovirus (CAdV) in owned dogs in Northern Italy. , 2014, Research in veterinary science.

[6]  M. Laurenson,et al.  Infectious canine hepatitis in red foxes (Vulpes vulpes) in the United Kingdom , 2013, Veterinary Record Case Reports.

[7]  A. Balboni,et al.  Molecular epidemiology of canine adenovirus type 1 and type 2 in free-ranging red foxes (Vulpes vulpes) in Italy. , 2013, Veterinary microbiology.

[8]  M. Beer,et al.  Development and validation of a triplex real-time PCR assay for the rapid detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of Bovine herpesvirus type 1. , 2011, Journal of virological methods.

[9]  V. Martella,et al.  Canine Adenoviruses and Herpesvirus , 2008, Veterinary Clinics of North America: Small Animal Practice.

[10]  Michael J. Chamberlain,et al.  Infectious Canine Hepatitis in a Gray Fox (Urocyon cinereoargenteus) , 2007, Journal of wildlife diseases.

[11]  V. Martella,et al.  Canine respiratory viruses. , 2007, Veterinary research.

[12]  M. Gompper,et al.  A SEROLOGIC ASSESSMENT OF EXPOSURE TO VIRAL PATHOGENS AND LEPTOSPIRA IN AN URBAN RACCOON (PROCYON LOTOR) POPULATION INHABITING A LARGE ZOOLOGICAL PARK , 2007, Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians.

[13]  A. Lorusso,et al.  Infectious canine hepatitis: An “old” disease reemerging in Italy , 2007, Research in Veterinary Science.

[14]  V. Martella,et al.  Detection of canine distemper virus in dogs by real-time RT-PCR. , 2006, Journal of virological methods.

[15]  D. Martineau,et al.  Use of Polymerase Chain Reaction and Immunohistochemistry for Detection of Canine Adenovirus Type 1 in Formalin-Fixed, Paraffin-Embedded Liver of Dogs with Chronic Hepatitis or Cirrhosis , 1998, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[16]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[17]  Z. Zhong,et al.  Detection and Differentiation of CAV-1 and CAV-2 by Polymerase Chain Reaction , 2004, Veterinary Research Communications.

[18]  I. Kiss,et al.  Infectious canine hepatitis: detection of canine adenovirus type 1 by polymerase chain reaction. , 1996, Acta veterinaria Hungarica.