Engineered proteins with desired specificity: DARPins, other alternative scaffolds and bispecific IgGs.

[1]  A. Skerra,et al.  Structure-guided engineering of Anticalins with improved binding behavior and biochemical characteristics for application in radio-immuno imaging and/or therapy. , 2014, Journal of structural biology.

[2]  Andrew Leaver-Fay,et al.  Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface , 2014, Nature Biotechnology.

[3]  David Baker,et al.  Computational design of a pH-sensitive IgG binding protein , 2013, Proceedings of the National Academy of Sciences.

[4]  Andreas Plückthun,et al.  From DARPins to LoopDARPins: novel LoopDARPin design allows the selection of low picomolar binders in a single round of ribosome display. , 2013, Journal of molecular biology.

[5]  A. Plückthun,et al.  Structural basis for eliciting a cytotoxic effect in HER2-overexpressing cancer cells via binding to the extracellular domain of HER2. , 2013, Structure.

[6]  M. Glaser,et al.  Three Methods for 18F Labeling of the HER2-Binding Affibody Molecule ZHER2:2891 Including Preclinical Assessment , 2013, The Journal of Nuclear Medicine.

[7]  K. Hahn,et al.  Knowledge-based design of a biosensor to quantify localized ERK activation in living cells. , 2013, Chemistry & biology.

[8]  C. Klein,et al.  Crystal Structure of an Anti-Ang2 CrossFab Demonstrates Complete Structural and Functional Integrity of the Variable Domain , 2013, PloS one.

[9]  A. Plückthun,et al.  DARPin-targeting of measles virus: unique bispecificity, effective oncolysis, and enhanced safety. , 2013, Molecular therapy : the journal of the American Society of Gene Therapy.

[10]  John A. Robinson,et al.  Conformation-Dependent Recognition of HIV gp120 by Designed Ankyrin Repeat Proteins Provides Access to Novel HIV Entry Inhibitors , 2013, Journal of Virology.

[11]  J. Andersen,et al.  A series of anti-CEA/anti-DOTA bispecific antibody formats evaluated for pre-targeting: comparison of tumor uptake and blood clearance. , 2013, Protein engineering, design & selection : PEDS.

[12]  Michaela Gebauer,et al.  Combinatorial design of an Anticalin directed against the extra-domain b for the specific targeting of oncofetal fibronectin. , 2013, Journal of molecular biology.

[13]  A. Plückthun,et al.  Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters , 2013, Proceedings of the National Academy of Sciences.

[14]  Wolfgang Schaefer,et al.  Progress in overcoming the chain association issue in bispecific heterodimeric IgG antibodies , 2012, mAbs.

[15]  S. Koide,et al.  Structural insights for engineering binding proteins based on non-antibody scaffolds. , 2012, Current opinion in structural biology.

[16]  Andreas Plückthun,et al.  Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries , 2012, Proceedings of the National Academy of Sciences.

[17]  Haruki Nakamura,et al.  Computer-aided antibody design , 2012, Protein engineering, design & selection : PEDS.

[18]  Timothy A. Whitehead,et al.  Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing , 2012, Nature Biotechnology.

[19]  Seungpyo Hong,et al.  Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering , 2012, Proceedings of the National Academy of Sciences.

[20]  Shohei Koide,et al.  Teaching an old scaffold new tricks: monobodies constructed using alternative surfaces of the FN3 scaffold. , 2012, Journal of molecular biology.

[21]  A. Plückthun,et al.  DARPins and other repeat protein scaffolds: advances in engineering and applications. , 2011, Current opinion in biotechnology.

[22]  M. Sliwkowski,et al.  A two-in-one antibody against HER3 and EGFR has superior inhibitory activity compared with monospecific antibodies. , 2011, Cancer cell.

[23]  John B Wojcik,et al.  Targeting the SH2-Kinase Interface in Bcr-Abl Inhibits Leukemogenesis , 2011, Cell.

[24]  Andrew Leaver-Fay,et al.  A Generic Program for Multistate Protein Design , 2011, PloS one.

[25]  Guy Georges,et al.  Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies , 2011, Proceedings of the National Academy of Sciences.

[26]  Steven M. Lewis,et al.  A biosensor generated via high throughput screening quantifies cell edge Src dynamics , 2011, Nature chemical biology.

[27]  Megan L. Murtaugh,et al.  A combinatorial approach to engineering a dual-specific metal switch antibody. , 2011, Biochemistry.

[28]  Timothy A. Whitehead,et al.  Computational Design of Proteins Targeting the Conserved Stem Region of Influenza Hemagglutinin , 2011, Science.

[29]  A. Plückthun,et al.  A Novel Fusion Toxin Derived from an EpCAM-Specific Designed Ankyrin Repeat Protein Has Potent Antitumor Activity , 2010, Clinical Cancer Research.

[30]  Andrew C. Chan,et al.  Therapeutic antibodies for autoimmunity and inflammation , 2010, Nature Reviews Immunology.

[31]  G. Himmler,et al.  Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu-binding sites and antibody properties. , 2010, Protein engineering, design & selection : PEDS.

[32]  Shohei Koide,et al.  A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain , 2010, Nature Structural &Molecular Biology.

[33]  Andreas Plückthun,et al.  Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size. , 2010, Cancer research.

[34]  C. Amemiya,et al.  Evolution of adaptive immune recognition in jawless vertebrates. , 2010, Seminars in immunology.

[35]  L. Bloom,et al.  FN3: a new protein scaffold reaches the clinic. , 2009, Drug discovery today.

[36]  Michael M. Schmidt,et al.  A modeling analysis of the effects of molecular size and binding affinity on tumor targeting , 2009, Molecular Cancer Therapeutics.

[37]  F. Peale,et al.  Variants of the Antibody Herceptin That Interact with HER2 and VEGF at the Antigen Binding Site , 2009, Science.

[38]  A. Skerra,et al.  High-affinity recognition of lanthanide(III) chelate complexes by a reprogrammed human lipocalin 2. , 2009, Journal of the American Chemical Society.

[39]  Tijana Z Grove,et al.  Ligand binding by repeat proteins: natural and designed. , 2008, Current opinion in structural biology.

[40]  G. Glazko,et al.  Diversity and Function of Adaptive Immune Receptors in a Jawless Vertebrate , 2005, Science.

[41]  A. Plückthun,et al.  Engineering novel binding proteins from nonimmunoglobulin domains , 2005, Nature Biotechnology.

[42]  Andreas Plückthun,et al.  Engineered proteins as specific binding reagents. , 2005, Current opinion in biotechnology.

[43]  Sophie E Jackson,et al.  A recurring theme in protein engineering: the design, stability and folding of repeat proteins. , 2005, Current opinion in structural biology.

[44]  D. Röthlisberger,et al.  Domain interactions in the Fab fragment: a comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stability. , 2005, Journal of molecular biology.

[45]  Chris T. Amemiya,et al.  Somatic diversification of variable lymphocyte receptors in the agnathan sea lamprey , 2004, Nature.

[46]  A. Plückthun,et al.  High-affinity binders selected from designed ankyrin repeat protein libraries , 2004, Nature Biotechnology.

[47]  Andreas Plückthun,et al.  Designing repeat proteins: modular leucine-rich repeat protein libraries based on the mammalian ribonuclease inhibitor family. , 2003, Journal of molecular biology.

[48]  Sophie E Jackson,et al.  The folding and design of repeat proteins: reaching a consensus. , 2003, Current opinion in structural biology.

[49]  Andrew C. R. Martin,et al.  Analysis of the antigen combining site: correlations between length and sequence composition of the hypervariable loops and the nature of the antigen. , 2003, Journal of molecular biology.

[50]  G. Denardo,et al.  Anti-HLA-DR/anti-DOTA diabody construction in a modular gene design platform: bispecific antibodies for pretargeted radioimmunotherapy. , 2001, Cancer biotherapy & radiopharmaceuticals.

[51]  S H Kim,et al.  Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii. , 2001, Biochemistry.

[52]  A. Kajava,et al.  Review: proteins with repeated sequence--structural prediction and modeling. , 2001, Journal of structural biology.

[53]  A. Beavil,et al.  Mutations in the DG Loop of Adenovirus Type 5 Fiber Knob Protein Abolish High-Affinity Binding to Its Cellular Receptor CAR , 1999, Journal of Virology.

[54]  G. Denardo,et al.  Antibody phage libraries for the next generation of tumor targeting radioimmunotherapeutics. , 1999, Clinical cancer research : an official journal of the American Association for Cancer Research.

[55]  Andreas Plückthun,et al.  Stable one-step technetium-99m labeling of His-tagged recombinant proteins with a novel Tc(I)–carbonyl complex , 1999, Nature Biotechnology.

[56]  A. Koide,et al.  The fibronectin type III domain as a scaffold for novel binding proteins. , 1998, Journal of molecular biology.

[57]  Camellia W. Adams,et al.  An efficient route to human bispecific IgG , 1998, Nature Biotechnology.

[58]  J. Wells,et al.  Stable heterodimers from remodeling the domain interface of a homodimer using a phage display library. , 1997, Journal of molecular biology.

[59]  L. Presta,et al.  'Knobs-into-holes' engineering of antibody CH3 domains for heavy chain heterodimerization. , 1996, Protein engineering.

[60]  V. Tolmachev,et al.  Methods for radiolabelling of monoclonal antibodies. , 2014, Methods in molecular biology.

[61]  J.-Y. Han,et al.  Evaluation of 99mTc-peptide-ZHER2:342 Affibody® molecule for in vivo molecular imaging. , 2014, The British journal of radiology.

[62]  G. Riethmüller Symmetry breaking: bispecific antibodies, the beginnings, and 50 years on. , 2012, Cancer immunity.

[63]  Sachdev S Sidhu,et al.  Simplified synthetic antibody libraries. , 2012, Methods in enzymology.

[64]  B. Nelson,et al.  Synthetic antibody libraries. , 2012, Methods in molecular biology.

[65]  Manuel Simon,et al.  Designed ankyrin repeat proteins (DARPins) from research to therapy. , 2012, Methods in enzymology.