Madurella mycetomatis Is Not Susceptible to the Echinocandin Class of Antifungal Agents

ABSTRACT Eumycetoma caused by Madurella mycetomatis is treated surgically and with high doses of ketoconazole. Therapeutic responses are poor, and recurrent infections are common. In search of therapeutic alternatives in the treatment of mycetoma, we determined the in vitro susceptibilities of M. mycetomatis isolates against caspofungin, anidulafungin, and micafungin. As a comparator fungus, Aspergillus fumigatus was used. Minimal effective concentrations (MECs) and MICs were assessed and compared to those of ketoconazole. M. mycetomatis isolates were not susceptible to the echinocandins.

[1]  I. Bakker-Woudenberg,et al.  The effects of antifungal agents to conidial and hyphal forms of Aspergillus fumigatus. , 2010, Medical mycology.

[2]  S. Katiyar,et al.  Role for Fks1 in the Intrinsic Echinocandin Resistance of Fusarium solani as Evidenced by Hybrid Expression in Saccharomyces cerevisiae , 2009, Antimicrobial Agents and Chemotherapy.

[3]  A. Zaas.  Echinocandins: a wealth of choice – how clinically different are they? , 2008, Current opinion in infectious diseases.

[4]  Clinical,et al.  Reference method for broth dilution antifungal susceptibility testing of filamentous fungi : Approved standard , 2008 .

[5]  D. Perlin.  Resistance to echinocandin-class antifungal drugs. , 2007, Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy.

[6]  M. Motyl,et al.  Caspofungin Susceptibility in Aspergillus and Non-Aspergillus Molds: Inhibition of Glucan Synthase and Reduction of β-d-1,3 Glucan Levels in Culture , 2006, Antimicrobial Agents and Chemotherapy.

[7]  A. van Belkum,et al.  Genotyping of Madurella mycetomatis by Selective Amplification of Restriction Fragments (Amplified Fragment Length Polymorphism) and Subtype Correlation with Geographical Origin and Lesion Size , 2005, Journal of Clinical Microbiology.

[8]  C. Selitrennikoff,et al.  Cryptococcus neoformans Resistance to Echinocandins: (1,3)β-Glucan Synthase Activity Is Sensitive to Echinocandins , 2005, Antimicrobial Agents and Chemotherapy.

[9]  A. van Belkum,et al.  Testing of the In Vitro Susceptibilities of Madurella mycetomatis to Six Antifungal Agents by Using the Sensititre System in Comparison with a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5- [(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Assay and a Modified NCCLS Method , 2005, Antimicrobial Agents and Chemotherapy.

[10]  A. van Belkum,et al.  In Vitro Susceptibilities of Madurella mycetomatis to Itraconazole and Amphotericin B Assessed by a Modified NCCLS Method and a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5- Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H- Tetrazolium Hydroxide (XTT) Assay , 2004, Antimicrobial Agents and Chemotherapy.

[11]  V. Paetznick,et al.  In Vitro Activity of Anidulafungin against Selected Clinically Important Mold Isolates , 2004, Antimicrobial Agents and Chemotherapy.

[12]  D. Delmer,et al.  A Microtiter-Based Fluorescence Assay for (1,3)-β-Glucan Synthases , 1997 .

[13]  D. Delmer,et al.  A microtiter-based fluorescence assay for (1,3)-beta-glucan synthases. , 1997, Analytical biochemistry.