Efficient and simple quantification of stratum corneum proteins on tape strippings by infrared densitometry

Background: The analysis of stratum corneum (SC) components is a widely accepted method to determine ‘skin health’ status or to follow the effects of topical treatments. These analytes are normally corrected to the amount of SC removed which can be determined gravimetrically or by extraction of SC proteins and their subsequent analysis. Unfortunately, this is a time consuming procedure and usually requires a laboratory. As a result there is a need for equipment that can be used in a clinical setting. Equally, a concurrent determination of total SC protein content and enzyme activity or any other analyte on the same tape stripping is difficult and also time consuming. Therefore a compact infrared densitometer was developed allowing a convenient and user friendly indirect measurement of SC protein content on tape strippings by optical absorption. As this is a non‐destructive technique, the tape strippings can subsequently be utilized for any other bioassay.

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