Angular dependent light scattering from multicellular spheroids.

We demonstrate that the effects of cell-cell contact and of changes in cell shape have only a minor effect on the angular distribution of light scattering from mammalian fibroblast cells. This result is important for the development of light scattering as a noninvasive tool for tissue diagnostics such as cancer detection. Changes in cell organization that are not accompanied by changes in internal cellular structure may not be measurable. On the other hand, changes in internal cellular structure should be measurable without interference from changes in overall cellular organization. The second major result of this work is that there are small but significant differences between light scattering of tumorigenic and nontumorigenic cells grown in a three-dimensional culture system. The cause of the differences in light scattering are likely due to the nontumorigenic cells arresting in the G1 phase of the cell cycle, while the tumorigenic cells continue to proliferate.

[1]  L Kunzschughart,et al.  Oncogene-associated transformation of rodent fibroblasts is accompanied by large morphologic and metabolic alterations. , 1995, Oncology reports.

[2]  J. Freyer,et al.  Differential regulation of cyclin-dependent kinase inhibitors in monolayer and spheroid cultures of tumorigenic and nontumorigenic fibroblasts. , 1998, Cancer research.

[3]  S. Lakhani,et al.  Diagnosis of breast cancer using elastic-scattering spectroscopy: preliminary clinical results. , 2000, Journal of biomedical optics.

[4]  Stefan Walenta,et al.  Metabolic Imaging in Multicellular Spheroids of Oncogene-transfected Fibroblasts , 2000, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[5]  Judith R. Mourant,et al.  Light scattering from cells: the contribution of the nucleus and the effects of proliferative status , 2000 .

[6]  Angela A. Eick,et al.  Mechanisms of light scattering from biological cells relevant to noninvasive optical-tissue diagnostics. , 1998, Applied optics.

[7]  R J Ott,et al.  Spectrophotometric assessment of pigmented skin lesions: methods and feature selection for evaluation of diagnostic performance. , 2000, Physics in medicine and biology.

[8]  L. Kunz-Schughart,et al.  Three‐dimensional cell culture induces novel proliferative and metabolic alterations associated with oncogenic transformation , 1996, International journal of cancer.

[9]  M Fitzmaurice,et al.  Endoscopic detection of dysplasia in patients with Barrett's esophagus using light-scattering spectroscopy. , 2000, Gastroenterology.

[10]  R Richards-Kortum,et al.  A pulsed finite-difference time-domain (FDTD) method for calculating light scattering from biological cells over broad wavelength ranges. , 2000, Optics express.

[11]  J. Schmitt,et al.  Optical scattering properties of soft tissue: a discrete particle model. , 1998, Applied optics.

[12]  J P Freyer,et al.  Characterizing Mammalian cells and cell phantoms by polarized backscattering fiber-optic measurements. , 2001, Applied optics.

[13]  A. Roggan,et al.  The effect of preparation technique on the optical parameters of biological tissue , 1999 .

[14]  J. Mourant,et al.  Elastic scattering spectroscopy as a diagnostic tool for differentiating pathologies in the gastrointestinal tract: preliminary testing. , 1996, Journal of biomedical optics.

[15]  W. Mueller‐Klieser.  Three-dimensional cell cultures: from molecular mechanisms to clinical applications. , 1997, American journal of physiology. Cell physiology.

[16]  I J Bigio,et al.  Spectroscopic diagnosis of bladder cancer with elastic light scattering , 1995, Lasers in surgery and medicine.

[17]  G. Zonios,et al.  Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo. , 1999, Applied optics.

[18]  J J ten Bosch,et al.  Optical properties of bovine muscle tissue in vitro; a comparison of methods , 1998 .

[19]  J P Freyer,et al.  Decreased mitochondrial function in quiescent cells isolated from multicellular tumor spheroids , 1998, Journal of cellular physiology.

[20]  A. Dunn,et al.  Light scattering from cells: finite-difference time-domain simulations and goniometric measurements. , 1999, Applied optics.

[21]  A H Hielscher,et al.  Evidence of intrinsic differences in the light scattering properties of tumorigenic and nontumorigenic cells , 1998, Cancer.

[22]  R. Knuechel,et al.  Multicellular spheroids: a three‐dimensional in vitro culture system to study tumour biology , 1998, International journal of experimental pathology.

[23]  R. Sutherland.  Cell and environment interactions in tumor microregions: the multicell spheroid model. , 1988, Science.