Simultaneous determination of trace levels of multiclass fungicides in natural waters by solid - phase microextraction - gas chromatography-tandem mass spectrometry.

A solvent-free method based on solid-phase microextraction (SPME) followed by gas chromatography-tandem mass spectrometry (GC-MS/MS) has been developed for the simultaneous determination of eleven fungicides at trace levels in different types of waters. Several experimental SPME parameters such as temperature, fibre coating, and SPME mode, were optimized in order to obtain the highest extraction efficiency. Under the optimal conditions, 100 °C, polyacrilate fibre (PA) and direct-immersion mode, the method was validated showing good linearity, repeatability and reproducibility. Recovery studies were carried out in four different real water matrices and at three concentration levels (20, 200 and 2000 ng L-1), with overall recovery values between 92 and 104% and relative standard deviations (RSD) about 10%. Limits of detection (LODs) at the low ng L-1 were obtained. The method demonstrated its suitability for the determination of fungicides in real water samples using external calibration for quantification purposes as well as for photodegradation studies at low concentration levels.

[1]  R. Cela,et al.  Multivariate optimization of the factors influencing the solid-phase microextraction of pyrethroid pesticides in water. , 2006, Journal of chromatography. A.

[2]  T. Albanis,et al.  Determination of fungicides in natural waters using solid-phase microextraction and gas chromatography coupled with electron-capture and mass spectrometric detection. , 2000, Journal of chromatography. A.

[3]  M. E. T. Padrón,et al.  Solid-phase microextraction of benzimidazole fungicides in environmental liquid samples and HPLC–fluorescence determination , 2007 .

[4]  F. Wang,et al.  Ionic liquid-based ultrasound-assisted emulsification microextraction coupled with high performance liquid chromatography for the determination of four fungicides in environmental water samples. , 2013, Talanta.

[5]  Chun Wang,et al.  Application of dispersion-solidification liquid-liquid microextraction for the determination of triazole fungicides in environmental water samples by high-performance liquid chromatography. , 2011, Journal of hazardous materials.

[6]  Bärbel Vieth,et al.  Residue analysis of 500 high priority pesticides: better by GC-MS or LC-MS/MS? , 2006, Mass spectrometry reviews.

[7]  G. Sindona,et al.  A solid-phase microextraction-gas chromatographic approach combined with triple quadrupole mass spectrometry for the assay of carbamate pesticides in water samples. , 2012, Journal of chromatography. A.

[8]  C. Ruepert,et al.  Pesticide residues in the aquatic environment of banana plantation areas in the North Atlantic Zone of Costa Rica , 2000 .

[9]  N. Menzies,et al.  Environmental Fate of Fungicides in Surface Waters of a Horticultural-Production Catchment in Southeastern Australia , 2012, Archives of Environmental Contamination and Toxicology.

[10]  Jing Cheng,et al.  Application of an ultrasound-assisted surfactant-enhanced emulsification microextraction method for the analysis of diethofencarb and pyrimethanil fungicides in water and fruit juice samples. , 2011, Analytica chimica acta.

[11]  Yongsheng Ji,et al.  Oxidized Multiwalled Carbon Nanotubes as an SPME Fiber Coating for Rapid LC–UV Analysis of Benzimidazole Fungicides in Water , 2009 .

[12]  C. Gonçalves,et al.  Multiresidue method for the simultaneous determination of four groups of pesticides in ground and drinking waters, using solid-phase microextraction-gas chromatography with electron-capture and thermionic specific detection. , 2002, Journal of Chromatography A.

[13]  J. C. D. Silva,et al.  Evaluation of the pesticide contamination of groundwater sampled over two years from a vulnerable zone in Portugal. , 2007, Journal of agricultural and food chemistry.

[14]  O. Jolliet,et al.  Life cycle impact assessment of pesticides on human health and ecosystems , 2002 .

[15]  F. Hernández,et al.  The role of GC-MS/MS with triple quadrupole in pesticide residue analysis in food and the environment , 2013 .

[16]  Lorraine Maltby,et al.  Fungicide risk assessment for aquatic ecosystems: importance of interspecific variation, toxic mode of action, and exposure regime. , 2009, Environmental science & technology.

[17]  R. Cela,et al.  Development of a solid-phase microextraction gas chromatography with microelectron-capture detection method for a multiresidue analysis of pesticides in bovine milk. , 2008, Analytica chimica acta.

[18]  V. Vilar,et al.  Photodegradation of multiclass fungicides in the aquatic environment and determination by liquid chromatography-tandem mass spectrometry , 2017, Environmental Science and Pollution Research.

[19]  M. Llompart,et al.  Determination of fungicides in white grape bagasse by pressurized liquid extraction and gas chromatography tandem mass spectrometry. , 2014, Journal of chromatography. A.