A Library of Monoclonal Antibodies to Torpedo Cholinergic Synaptosomes

Abstract: A library of monoclonal antibodies was generated to the cholinergic synaptosome. The immunogen was a preparation of highly purified synaptosomes from Torpedo electric organ. One hundred forty‐one hybridoma cell lines were generated from the fusion of a single mouse. Tests reveal these cells produce antibodies with a vast range of neuronal specificities. The initial screen for specificity of antibody production was solid phase radioimmune binding to the original, highly purified synaptosome preparation. Subsequent tissue specificity tests have indicated that most antibodies are synaptosome‐specific amongst the fish tissues tested: brain, liver, and purified synaptic vesicles. Less than 11% cross‐react with liver. Many antibodies cross‐react with frog and rat CNS. Localization within the frog and rat nervous tissue has revealed a vast array of antibody staining patterns. Some antibodies stain in a synaptic fashion. A few stain a restricted set of mammalian CNS neurons. Others define a broader set of CNS neurons. These Torpedo antibodies promise to be valuable probes with which to describe the molecular cell biology of the nervous system, of neurons in general, and of cholinergic neurons in particular.

[1]  R. Kelly,et al.  A synaptic vesicle antigen is restricted to the junctional region of the presynaptic plasma membrane. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[2]  R. Kelly,et al.  A highly antigenic proteoglycan-like component of cholinergic synaptic vesicles. , 1983, The Journal of biological chemistry.

[3]  S. Hockfield,et al.  Monoclonal antibodies distinguish antigenically discrete neuronal types in the vertebrate central nervous system. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[4]  B. Zipser.  Complete distribution patterns of neurons with characteristic antigens in the leech central nervous system , 1982, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[5]  R. B. Kelly,et al.  Partial purification of presynaptic plasma membrane by immunoadsorption , 1982, The Journal of cell biology.

[6]  V. P. Whittaker,et al.  Identification of a Cholinergic‐Specific Antigen Chol‐1 as a Ganglioside , 1982, Journal of neurochemistry.

[7]  A. Matus,et al.  Monoclonal antibodies identify novel neural antigens. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[8]  T. Jessell,et al.  A monoclonal antibody defining antigenic determinants on subpopulations of mammalian neurones and Trypanosoma cruzi parasites , 1982, Nature.

[9]  S. Furukawa,et al.  Monoclonal antibody to acetylcholine receptor: cell line established from thymus of patient with Myasthenia gravis. , 1982, Science.

[10]  L. Sternberger,et al.  Neurotypy: regional individuality in rat brain detected by immunocytochemistry with monoclonal antibodies. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[11]  L. Reichardt,et al.  Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue , 1981, The Journal of cell biology.

[12]  R. McKay,et al.  Monoclonal antibodies distinguish identifiable neurones in the leech , 1981, Nature.

[13]  C. Barnstable.  Monoclonal antibodies which recognize different cell types in the rat retina , 1980, Nature.

[14]  V. Lennon,et al.  Myasthenia gravis induced by monoclonal antibodies to acetylcholine receptors , 1980, Nature.

[15]  S. Salpeter,et al.  Organization of acetylcholine receptors in quick-frozen, deep-etched, and rotary-replicated Torpedo postsynaptic membrane , 1979, The Journal of cell biology.

[16]  V. P. Whittaker,et al.  Identification of vesiculin as a glycosaminoglycan , 1978, Brain Research.

[17]  D. Michaelson,et al.  INDUCED ACETYLCHOLINE RELEASE FROM ACTIVE PURELY CHOLINERGIC TORPEDO SYNAPTOSOMES 1 , 1978, Journal of neurochemistry.

[18]  N. Morel,et al.  Isolation of pure cholinergic nerve endings from Torpedo electric organ. Evaluation of their metabolic properties , 1977, The Journal of cell biology.

[19]  N. Morel,et al.  Isolation of pure cholinergic nerve endings from the electric organ of Torpedo marmorata. , 1976, The Biochemical journal.

[20]  J. Patrick,et al.  Autoimmune Response to Acetylcholine Receptor , 1973, Science.

[21]  N. Klinman.  THE MECHANISM OF ANTIGENIC STIMULATION OF PRIMARY AND SECONDARY CLONAL PRECURSOR CELLS , 1972, The Journal of experimental medicine.

[22]  M. Karnovsky,et al.  A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES , 1964, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[23]  K. Barald.  Monoclonal Antibodies to Embryonic Neurons , 1982 .

[24]  V. Oi.  Immunoglobulin producing hybrid cell lines , 1980 .

[25]  L. Reichardt,et al.  Biochemical studies on the development of primary sympathetic neurons in cell culture. , 1976, Cold Spring Harbor symposia on quantitative biology.