Comparison of the myelin period of peripheral and central origin by electron microscopy.

The repeating radial periods of some peripheral and central nervous system myelin sheaths in albino rats were compared in an electron microscopic study. Essentially the peripheral myelin period appeared to be larger than the central by about 10%. This result confirmed earlier wide-angle X-ray diffraction data. Direction of sectioning was observed to affect the myelin period, probably proportionally to the hardness of the embedded tissue blocks. Microscopical procedures also tended to increase the spreading of the measurements provided the myelin period was assumed to be similar within the same part of the nervous system in the same animal species. This was elucidated by comparing differences obtained in the same and separate microscopy sessions. Possible causes of error were discussed.

[1]  I. Brody.  The keratinization of epidermal cells of normal guinea pig skin as revealed by electron microscopy , 1959 .

[2]  J. Pieters,et al.  ACCURATE DETERMINATION OF MAGNIFICATION IN THE ELECTRON MICROSCOPE. , 1964, Journal of ultrastructure research.

[3]  H. Maturana.  The Fine Anatomy of the Optic Nerve of Anurans—An Electron Microscope Study , 1960, The Journal of biophysical and biochemical cytology.

[4]  H. Fernández-morán,et al.  ELECTRON MICROSCOPE AND LOW-ANGLE X-RAY DIFFRACTION STUDIES OF THE NERVE MYELIN SHEATH , 1957, The Journal of biophysical and biochemical cytology.

[5]  J. B. Finean,et al.  ELECTRON MICROSCOPE AND X-RAY DIFFRACTION STUDIES OF THE EFFECTS OF DEHYDRATION ON THE STRUCTURE OF NERVE MYELIN , 1960, The Journal of biophysical and biochemical cytology.

[6]  J. Finean.  Further observations on the structure of myelin. , 1953, Experimental cell research.

[7]  M. Ments,et al.  Numerical computation of the constants of magnetic electron lenses , 1950 .

[8]  Robert L. Schultz,et al.  Fixation of the central nervous system for electron microscopy by aldehyde perfusion , 1965 .

[9]  R. Schultz,et al.  Plasma Membrane Apposition in the Central Nervous System after Aldehyde Perfusion , 1964, Nature.

[10]  E. Reynolds.  THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY , 1963, The Journal of cell biology.

[11]  F. S. Sjostrand,et al.  Submicroscopic organisation of some layered lipoprotein structures (nerve myelin, retinal rods, and chloroplasts). , 1953, Experimental cell research.

[12]  A. Peters.  Plasma membrane contacts in the central nervous system. , 1962, Journal of anatomy.

[13]  A. Peters,et al.  The Structure of Myelin Sheaths in the Central Nervous System of Xenopus laevis (Daudin) , 1960, The Journal of biophysical and biochemical cytology.

[14]  J. Finean,et al.  THE LIPID COMPOSITION OF MYELIN FROM BRAIN AND PERIPHERAL NERVE , 1965, Journal of neurochemistry.

[15]  W. R. Buckland,et al.  Statistical Theory and Methodology in Science and Engineering. , 1960 .