Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol

[1]  G. Raposo,et al.  Extracellular vesicles shuffling intercellular messages: for good or for bad. , 2015, Current opinion in cell biology.

[2]  Claudia Mongini,et al.  Emerging Roles of Exosomes in Normal and Pathological Conditions: New Insights for Diagnosis and Therapeutic Applications , 2015, Front. Immunol..

[3]  Mukesh Verma,et al.  Extracellular vesicles: potential applications in cancer diagnosis, prognosis, and epidemiology , 2015, BMC Clinical Pathology.

[4]  Yanmei Xu,et al.  Exosomes: Novel Biomarkers for Clinical Diagnosis , 2015, TheScientificWorldJournal.

[5]  Joshua N Leonard,et al.  Therapeutic applications of extracellular vesicles: clinical promise and open questions. , 2015, Annual review of pharmacology and toxicology.

[6]  Matt Trau,et al.  Analysis of exosome purification methods using a model liposome system and tunable-resistive pulse sensing , 2015, Scientific Reports.

[7]  Cecilia Lässer.  Exosomes in diagnostic and therapeutic applications: biomarker, vaccine and RNA interference delivery vehicle , 2015, Expert opinion on biological therapy.

[8]  C. Théry,et al.  Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. , 2014, Annual review of cell and developmental biology.

[9]  Jayakumar Rajadas,et al.  Exosomes as immunotheranostic nanoparticles. , 2014, Clinical therapeutics.

[10]  Olivier Elemento,et al.  Double-stranded DNA in exosomes: a novel biomarker in cancer detection , 2014, Cell Research.

[11]  J. Lötvall,et al.  The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles , 2014, Journal of extracellular vesicles.

[12]  Winston Patrick Kuo,et al.  Current methods for the isolation of extracellular vesicles , 2013, Biological chemistry.

[13]  A. Seifalian,et al.  Exosomes as nano-theranostic delivery platforms for gene therapy. , 2013, Advanced drug delivery reviews.

[14]  Graça Raposo,et al.  Extracellular vesicles: Exosomes, microvesicles, and friends , 2013, The Journal of cell biology.

[15]  J. Rak,et al.  Extracellular Vesicles – Biomarkers and Effectors of the Cellular Interactome in Cancer , 2013, Front. Pharmacol..

[16]  J. Lötvall,et al.  Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes , 2013, Journal of extracellular vesicles.

[17]  Lynne T. Bemis,et al.  Standardization of sample collection, isolation and analysis methods in extracellular vesicle research , 2013, Journal of extracellular vesicles.

[18]  R. Setterquist,et al.  Exosomes: current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials. , 2012, Biochimica et biophysica acta.

[19]  Winston Patrick Kuo,et al.  Impact of Biofluid Viscosity on Size and Sedimentation Efficiency of the Isolated Microvesicles , 2012, Front. Physio..

[20]  Richard J Simpson,et al.  Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. , 2012, Methods.

[21]  C. Théry,et al.  Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation , 2012, Journal of extracellular vesicles.

[22]  György Nagy,et al.  Cellular and Molecular Life Sciences REVIEW Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles , 2022 .

[23]  M. Wood,et al.  Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes , 2011, Nature Biotechnology.

[24]  S. Mathivanan,et al.  Exosomes: extracellular organelles important in intercellular communication. , 2010, Journal of proteomics.

[25]  Graça Raposo,et al.  Exosomes--vesicular carriers for intercellular communication. , 2009, Current opinion in cell biology.

[26]  J. Rak,et al.  Microvesicles: Messengers and mediators of tumor progression , 2009, Cell cycle.

[27]  C. Hess,et al.  Polymorphonuclear Neutrophil-Derived Ectosomes Interfere with the Maturation of Monocyte-Derived Dendritic Cells1 , 2008, The Journal of Immunology.

[28]  J. Meldolesi,et al.  Enlargeosome Traffic: Exocytosis Triggered by Various Signals Is Followed by Endocytosis, Membrane Shedding or Both , 2007, Traffic.

[29]  Aled Clayton,et al.  Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids , 2006, Current protocols in cell biology.

[30]  J. Kestin,et al.  Viscosity of Liquid Water in the Range - 8 C to 150 C, , 1978 .

[31]  N. Anderson,et al.  Analytical techniques for cell fractions. 8. Analytical differential centrifugation in angle-head rotors. , 1968, Analytical Biochemistry.

[32]  K. Bryniarski,et al.  Exosomes as mediators of intercellular communication: clinical implications. , 2015, Polskie Archiwum Medycyny Wewnetrznej.

[33]  Standard Guide for Measurement of Particle Size Distribution of Nanomaterials in Suspension by Nanoparticle Tracking Analysis ( NTA ) 1 , 2015 .

[34]  Bernd Giebel,et al.  Exosomes: small vesicles participating in intercellular communication. , 2012, The international journal of biochemistry & cell biology.

[35]  V. Schumaker,et al.  Theory of differential centrifugation in angle-head rotors. , 1969, Analytical biochemistry.

[36]  N. Anderson,et al.  Analytical techniques for cell fractions , 1969 .

[37]  N. Anderson.  An introduction to particle separations in zonal centrifuges. , 1966, National Cancer Institute monograph.

[38]  J. Philpot.  The Ultracentrifuge , 1943, Nature.

[39]  T. Svedberg.  The Ultra-Centrifuge and the Study of High-Molecular Compounds , 1937, Nature.