Mutations of the Ki-ras oncogene in carcinoma of the endometrium.

Mutations of the Ki-ras oncogene in endometrial carcinoma have been reported in Japan, but the prevalence and clinical significance of such mutations in the United States remains unclear. DNA extracted from paraffin sections of 112 carcinomas of the endometrium was amplified by the polymerase chain reaction with mismatched primers that generated a BstNI recognition site with the wild-type codon 12. Loss of this recognition site indicating Ki-ras codon 12 mutations was observed in 13 tumors (11.6%), including 11 endometrioid carcinomas, one undifferentiated carcinoma, and one carcinosarcoma. None of 17 papillary serous-clear cell carcinomas contained Ki-ras codon 12 mutations. These mutations were confirmed and characterized by direct sequencing. We found no evidence of correlation of the presence of Ki-ras mutations with stage, grade, depth of invasion, or clinical outcome. Our results indicate that Ki-ras oncogene mutations in carcinoma of the endometrium may be less prevalent in the United States than in Japan.

[1]  K. Crabtree.  Genetics, Natural History, Tumor Spectrum, and Pathology of Hereditary Nonpolyposis Colorectal Cancer: An Updated Review , 1994 .

[2]  J. Hancock,et al.  Activation of Raf as a result of recruitment to the plasma membrane. , 1994, Science.

[3]  L. Aaltonen,et al.  Microsatellite instability is associated with tumors that characterize the hereditary non-polyposis colorectal carcinoma syndrome. , 1993, Cancer research.

[4]  J. Chang,et al.  Mutation analysis of K-ras oncogenes in gastroenterologic cancers by the amplified created restriction sites method. , 1993, American journal of clinical pathology.

[5]  S. Goodman,et al.  K-ras oncogene activation in adenocarcinoma of the human pancreas. A study of 82 carcinomas using a combination of mutant-enriched polymerase chain reaction analysis and allele-specific oligonucleotide hybridization. , 1993, The American journal of pathology.

[6]  M. Birrer,et al.  p53 and Ki-ras gene mutations in epithelial ovarian neoplasms. , 1993, Cancer research.

[7]  H. Iwasaki,et al.  Characteristics of cloned cells of mixed müllerian tumor of the human uterus carcinoma cells showing myogenic differentiation in vitro , 1993, Cancer.

[8]  M. Inoue,et al.  Alterations of the p53 tumor suppressor gene and its association with activation of the c-K-ras-2 protooncogene in premalignant and malignant lesions of the human uterine endometrium. , 1993, Cancer research.

[9]  J. Koulos,et al.  Relationships between c-Ki-ras mutations, HPV types, and prognostic indicators in invasive endocervical adenocarcinomas. , 1993, Gynecologic oncology.

[10]  Y. Hirai,et al.  Studies on ras oncogene activation in endometrial carcinoma , 1993, Gynecologic oncology.

[11]  A. Berchuck,et al.  Mutations of the Ki-ras oncogene in endometrial carcinoma. , 1992, American journal of obstetrics and gynecology.

[12]  S. Nasim,et al.  Clinical implications of K-ras mutations in malignant epithelial tumors of the endometrium. , 1992, Cancer research.

[13]  M. Inoue,et al.  K-ras activation in premalignant and malignant epithelial lesions of the human uterus. , 1991, Cancer research.

[14]  M. Inoue,et al.  K-ras activation occurs frequently in mucinous adenocarcinomas and rarely in other common epithelial tumors of the human ovary. , 1991, The American journal of pathology.

[15]  M. Cauchi,et al.  Point mutations at codon 12 of C-K-ras in human endometrial carcinomas. , 1990, Cancer letters.

[16]  R. Kurman,et al.  The Significance of Epithelial Differences in Mixed Mesodermal Tumors of the Uterus: A Clinicopathologic and Immunohistochemical Study , 1990, The American journal of surgical pathology.

[17]  E. E. Gresch.  Genetic Alterations During Colorectal-Tumor Development , 1989 .

[18]  Joyce Bos.  ras oncogenes in human cancer: a review. , 1989, Cancer research.

[19]  G. Riou,et al.  Somatic deletions and mutations of c-Ha-ras gene in human cervical cancers. , 1988, Oncogene.

[20]  A. Yajima,et al.  Endometrial carcinoma in miyagi prefecture, Japan: Histopathologic analysis of a cancer registry‐based series and comparison with cases in American women , 1982, Cancer.

[21]  今村 利朗.  Chromosomal deletions and K-ras gene mutations in human endometrial carcinomas , 1994 .