New Electrostatic Phase Plate for Phase-Contrast Transmission Electron Microscopy and Its Application for Wave-Function Reconstruction

Abstract A promising novel type of electrostatic phase plate for transmission electron microscopy (TEM) is presented. The phase plate consists of a single microcoaxial cable-like rod with its electrode exposed to the undiffracted electrons. The emerging field is used to shift the phase of the undiffracted electrons with respect to diffracted electrons. The design overcomes the drawback of the spatial frequency-blocking ring electrode of the Boersch phase plate. First, experimental phase-contrast images are presented for PbSe and Pt nanoparticles with clearly varying phase contrast, which depends on the applied voltage and resulting phase shift of the unscattered electrons. With the new phase-plate design, we show for the first time the reconstruction of an object wave function based on a series of only three experimental phase-contrast TEM images obtained with an electrostatic phase plate.

[1]  A. Thust,et al.  Focal-series reconstruction in HRTEM: simulation studies on non-periodic objects , 1996 .

[2]  F. Tseng,et al.  Phase TEM for biological imaging utilizing a Boersch electrostatic phase plate: theory and practice. , 2009, Journal of electron microscopy.

[3]  D. Van dyck,et al.  Experiments on inelastic electron holography. , 2006, Ultramicroscopy.

[4]  Hannes Lichte,et al.  Electron holography approaching atomic resolution , 1986 .

[5]  R. Schröder,et al.  Object wave reconstruction by phase-plate transmission electron microscopy. , 2010, Ultramicroscopy.

[6]  Über die Kontraste von Atomen im Elektronenmikroskop , 1947 .

[7]  K. Nagayama,et al.  Complex Observation in Electron Microscopy. II. Direct Visualization of Phases and Amplitudes of Exit Wave Functions , 2001 .

[8]  K. Nagayama,et al.  Transmission electron microscopy with Zernike phase plate. , 2001, Ultramicroscopy.

[9]  D. Alloyeau,et al.  Imaging of soft and hard materials using a Boersch phase plate in a transmission electron microscope , 2010 .

[10]  F. Zernike Phase contrast, a new method for the microscopic observation of transparent objects , 1942 .

[11]  K. M. Zinn,et al.  Transmission electron microscopy. , 1973, International ophthalmology clinics.

[12]  R. Schröder,et al.  Fabrication of a Boersch phase plate for phase contrast imaging in a transmission electron microscope , 2006 .

[13]  R. Schröder,et al.  Optimizing phase contrast in transmission electron microscopy with an electrostatic (Boersch) phase plate. , 2007, Ultramicroscopy.

[14]  R. Shigemoto,et al.  Tuning of the Zernike phase-plate for visualization of detailed ultrastructure in complex biological specimens. , 2009, Journal of structural biology.

[15]  K. Downing,et al.  Design of a microfabricated, two-electrode phase-contrast element suitable for electron microscopy. , 2006, Ultramicroscopy.

[16]  C. Koch A flux-preserving non-linear inline holography reconstruction algorithm for partially coherent electrons. , 2008, Ultramicroscopy.