Binding and regulation of cellular functions by monoclonal antibodies against human tumor necrosis factor receptors

The present study was undertaken to further characterize the interaction of monoclonal antibodies (mAbs) against tumor necrosis factor (TNF) receptors with different targets, and to assess their ability to influence TNF effects on U937 and human endothelial cell (HEC) functions. Actions of recombinant TNF-alpha on U937 and HEC were effectively inhibited by Htr-5 and Utr-1, and to a greater extent by a combination of both mAbs. These observations indicate that TNF interaction with antigenically different components of membrane receptors (p55 and p75) represents a crucial step in transduction of signals for TNF toxicity against U937 and TNF activation of HEC functions.

[1]  C. Smith,et al.  A receptor for tumor necrosis factor defines an unusual family of cellular and viral proteins. , 1990, Science.

[2]  H. Tabuchi,et al.  Molecular cloning and expression of the human 55 kd tumor necrosis factor receptor , 1990, Cell.

[3]  G. Wong,et al.  Molecular cloning and expression of a receptor for human tumor necrosis factor , 1990, Cell.

[4]  H. Loetscher,et al.  Characterization of binding and biological effects of monoclonal antibodies against a human tumor necrosis factor receptor , 1990, The Journal of experimental medicine.

[5]  H. Hohmann,et al.  Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha). , 1989, The Journal of biological chemistry.

[6]  A. Waage,et al.  Cytokine regulation of interleukin 6 production by human endothelial cells. , 1989, Cellular immunology.

[7]  B. Beutler,et al.  The role of cachectin/TNF in endotoxic shock and cachexia. , 1988, Immunology today.

[8]  B. Aggarwal,et al.  Receptor Binding and Activation of Polymorphonuclear Neutrophils by Tumor Necrosis Factor‐Alpha , 1987, Journal of leukocyte biology.

[9]  G. Wong,et al.  Tumour necrosis factors α and β inhibit virus replication and synergize with interferons , 1986, Nature.

[10]  Bharat B. Aggarwal,et al.  Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin , 1984, Nature.

[11]  G. Nicolson,et al.  Immunoaffinity isolation of membrane antigens with biotinylated monoclonal antibodies and immobilized streptavidin matrices. , 1984, Journal of immunological methods.

[12]  G. Striker,et al.  Factors influencing endothelial cell proliferation in vitro , 1978, Journal of cellular physiology.