Overproduction of transposon Tn10-encoded tetracycline resistance protein results in cell death and loss of membrane potential

High-level expression of the Tn10 tetracycline resistance protein TetA in Escherichia coli caused partial collapse of the membrane potential, arrest of growth, and killing of the cells. Since alpha-methylglucoside transport was not affected, the overproduced TetA protein may cause not destruction of membrane structure but rather unrestricted translocation of protons and/or ions across the membrane.

[1]  C. Beck,et al.  Tetracycline diffusion through phospholipid bilayers and binding to phospholipids , 1984, Antimicrobial Agents and Chemotherapy.

[2]  I. Chopra,et al.  Reduced expression of Tn 10-mediated tetracycline resistance in Escherichia coli containing more than one copy of the transposon. , 1981, Journal of general microbiology.

[3]  T. Nguyen,et al.  Multicopy Tn10 tet plasmids confer sensitivity to induction of tet gene expression , 1983, Journal of bacteriology.

[4]  P. Postma,et al.  Phosphoenolpyruvate:carbohydrate phosphotransferase system of bacteria. , 1985, Microbiological reviews.

[5]  S. Levy,et al.  Detection of an inducible membrane protein associated with R-factor-mediated tetracycline resistance. , 1974, Biochemical and biophysical research communications.

[6]  E. Padan,et al.  Biosynthesis of the lactose permease in Escherichia coli minicells and effect of carrier amplification on cell physiology. , 1983, The Journal of biological chemistry.

[7]  H. Moyed,et al.  Mutations in multicopy Tn10 tet plasmids that confer resistance to inhibitory effects of inducers of tet gene expression , 1983, Journal of bacteriology.

[8]  T. Foster,et al.  Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K-12 chromosome , 1975, Journal of bacteriology.

[9]  J. Barbé,et al.  A multifunctional gene (tetR) controls Tn10-encoded tetracycline resistance , 1982, Journal of bacteriology.

[10]  P. Postma,et al.  Phosphoenolpyruvate:carbohydrate phosphotransferase system of bacteria , 1985 .

[11]  S. Levy,et al.  Plasmid-determined tetracycline resistance involves new transport systems for tetracycline , 1978, Nature.

[12]  A. M. George,et al.  Gene in the major cotransduction gap of the Escherichia coli K-12 linkage map required for the expression of chromosomal resistance to tetracycline and other antibiotics , 1983, Journal of bacteriology.

[13]  F. Harold,et al.  Role of an electrical potential in the coupling of metabolic energy to active transport by membrane vesicles of Escherichia coli. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[14]  W. Reznikoff,et al.  Overlapping divergent promoters control expression of Tn10 tetracycline resistance. , 1983, Gene.

[15]  J. McCarthy,et al.  Proton conduction by subunit a of the membrane‐bound ATP synthase of Escherichia coli revealed after induced overproduction. , 1985, The EMBO journal.

[16]  B. Rak,et al.  Regulation of the bgl operon of Escherichia coli by transcriptional antitermination. , 1988, The EMBO journal.

[17]  W. Reznikoff,et al.  Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10 , 1979, Journal of bacteriology.

[18]  D. Botstein,et al.  Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. , 1975, Journal of molecular biology.