The influence of psychrophilic and mesophilic start-up temperature on microbial fuel cell system performance

The successful scale-up of microbial fuel cells (MFC) for wastewater treatment in temperate regions requires the development of reactor systems that are robust to seasonal fluctuations and are energy efficient. Therefore, as part of this study, a MFC was acclimated for operation over a 8–35 °C temperature range. Employing single chamber air cathode MFCs, system performance was initially examined at three different operating temperatures (10 °C, 20 °C and 35 °C). At each temperature a maximum steady-state voltage of 0.49 ± 0.02 V was achieved after an operational period of 47 weeks, with the time to reach steady-state voltage being dependent on acclimation temperature. The highest COD removal rates of 2.98 g COD L−1 d−1 were produced in the 35 °C reactor but coulombic efficiencies (CEs) were found to be significantly higher at psychrophilic temperatures. Acclimation at different temperatures was found to a have a significant effect on the dynamic selection of psychrophilic, psychrotolerant and mesophilic anode respiring bacteria (ARB). This was demonstrated by subsequent static temperature studies which showed that only the 20 °C acclimated reactor was capable of optimal operation over a 10–35 °C temperature range and this was facilitated by the activity of psychrotolerant microorganisms. These results show that MFC systems may be successfully adapted for use in temperate climates to provide a stable method of bioenergy recovery and organic contaminant removal as part of wastewater treatment processes.

[1]  D. R. Bond,et al.  Electrode-Reducing Microorganisms That Harvest Energy from Marine Sediments , 2002, Science.

[2]  Byung Hong Kim,et al.  Continuous electricity production from artificial wastewater using a mediator-less microbial fuel cell. , 2006, Bioresource technology.

[3]  Bruce E Logan,et al.  Effectiveness of domestic wastewater treatment using microbial fuel cells at ambient and mesophilic temperatures. , 2010, Bioresource technology.

[4]  W. Verstraete,et al.  Evaluation of nested PCR-DGGE (denaturing gradient gel electrophoresis) with group-specific 16S rRNA primers for the analysis of bacterial communities from different wastewater treatment plants. , 2002, FEMS microbiology ecology.

[5]  K. Scott,et al.  Effect of temperature on the performance of microbial fuel cells , 2010 .

[6]  W. Verstraete,et al.  Initial community evenness favours functionality under selective stress , 2009, Nature.

[7]  G Lettinga,et al.  Challenge of psychrophilic anaerobic wastewater treatment. , 2001, Trends in biotechnology.

[8]  L. Raskin,et al.  Diversity and dynamics of microbial communities in engineered environments and their implications for process stability. , 2003, Current opinion in biotechnology.

[9]  C. Buisman,et al.  Towards practical implementation of bioelectrochemical wastewater treatment. , 2008, Trends in biotechnology.

[10]  Stefano Freguia,et al.  Microbial fuel cells: methodology and technology. , 2006, Environmental science & technology.

[11]  In Seop Chang,et al.  Enrichment, performance, and microbial diversity of a thermophilic mediatorless microbial fuel cell. , 2006, Environmental science & technology.

[12]  W. Verstraete,et al.  Quantifying Community Dynamics of Nitrifiers in Functionally Stable Reactors , 2007, Applied and Environmental Microbiology.

[13]  Hong Liu,et al.  Production of electricity from acetate or butyrate using a single-chamber microbial fuel cell. , 2005, Environmental science & technology.

[14]  D. R. Bond,et al.  Potential Role of a Novel Psychrotolerant Member of the Family Geobacteraceae, Geopsychrobacter electrodiphilus gen. nov., sp. nov., in Electricity Production by a Marine Sediment Fuel Cell , 2004, Applied and Environmental Microbiology.

[15]  V. O’Flaherty,et al.  Psychrophilic and mesophilic anaerobic digestion of brewery effluent: a comparative study. , 2006, Water research.

[16]  Grietje Zeeman,et al.  Treatment of domestic sewage in a two-step anaerobic filter/anaerobic hybrid system at low temperature. , 2002, Water research.

[17]  Alan J Guwy,et al.  Modular tubular microbial fuel cells for energy recovery during sucrose wastewater treatment at low organic loading rate. , 2010, Bioresource technology.

[18]  B. Silverman,et al.  The contribution of species richness and composition to bacterial services , 2005, Nature.

[19]  P. Parameswaran,et al.  Evaluation of energy-conversion efficiencies in microbial fuel cells (MFCs) utilizing fermentable and non-fermentable substrates. , 2008, Water research.

[20]  W. Verstraete,et al.  Biofuel Cells Select for Microbial Consortia That Self-Mediate Electron Transfer , 2004, Applied and Environmental Microbiology.

[21]  P. de Vos,et al.  Failure of the ammonia oxidation process in two pharmaceutical wastewater treatment plants is linked to shifts in the bacterial communities , 2005, Journal of applied microbiology.

[22]  Kees Roest,et al.  Community analysis of a full-scale anaerobic bioreactor treating paper mill wastewater. , 2005, Systematic and applied microbiology.

[23]  Willy Verstraete,et al.  How to get more out of molecular fingerprints: practical tools for microbial ecology. , 2008, Environmental microbiology.

[24]  Willy Verstraete,et al.  Microbial Resource Management: The Road To Go for Environmental Biotechnology , 2007 .

[25]  V. O’Flaherty,et al.  Long-term, high-rate anaerobic biological treatment of whey wastewaters at psychrophilic temperatures. , 2006, Bioresource technology.

[26]  M. Ghangrekar,et al.  Performance of microbial fuel cell subjected to variation in pH, temperature, external load and substrate concentration. , 2009, Bioresource technology.

[27]  B. Logan,et al.  Electricity-producing bacterial communities in microbial fuel cells. , 2006, Trends in microbiology.

[28]  Hang-Sik Shin,et al.  Variation of power generation at different buffer types and conductivities in single chamber microbial fuel cells. , 2010, Biosensors & bioelectronics.

[29]  Richard M. Dinsdale,et al.  Development of a tubular microbial fuel cell (MFC) employing a membrane electrode assembly cathode , 2009 .

[30]  J. Puhakka,et al.  Psychrotolerant and microaerophilic bacteria in boreal groundwater. , 2002, FEMS microbiology ecology.

[31]  B. Jørgensen,et al.  Community Size and Metabolic Rates of Psychrophilic Sulfate-Reducing Bacteria in Arctic Marine Sediments , 1999, Applied and Environmental Microbiology.

[32]  Grietje Zeeman,et al.  The role of anaerobic digestion of domestic sewage in closing the water and nutrient cycle at community level , 1999 .