Spatial analysis of a microbead using surface plasmon resonance coupled fluorescence

Surface plasmon resonance (SPR) coupled fluorescence uses an evanescent electromagnetic field to excite fluorophores in the vicinity of surface. We investigated the influence of enhanced evanescent fields at SPR on the induced fluorescence intensity. The system of this study is based on angle scanning with a half-cylinder prism (SF10) and dual motorized rotation stages to observe the correlation between the evanescent fields and fluorescent intensity of microbeads. With this system, emission from fluorophores only exists in close proximity to the surface of the microbead. The results show that evanescent fields produced at SPR provide more sensitive fluorescence images compared to those measured at a total internal reflection angle.

[1]  Wolfgang Knoll,et al.  Surface-Plasmon Field-Enhanced Fluorescence Spectroscopy , 2000 .

[2]  Michael J. Natan,et al.  SURFACE PLASMON RESONANCE OF AU COLLOID-MODIFIED AU FILMS : PARTICLE SIZE DEPENDENCE , 1999 .

[3]  W. Knoll,et al.  Investigating the kinetics of DNA-DNA and PNA-DNA interactions using surface plasmon resonance-enhanced fluorescence spectroscopy. , 2001, Biosensors & bioelectronics.

[4]  Lin He,et al.  Colloidal Au-Enhanced Surface Plasmon Resonance for Ultrasensitive Detection of DNA Hybridization , 2000 .

[5]  A. Gast,et al.  Surface Plasmon Resonance/Surface Plasmon Enhanced Fluorescence: An Optical Technique for the Detection of Multicomponent Macromolecular Adsorption at the Solid/Liquid Interface , 2002 .

[6]  Shiping Fang,et al.  Determination of ribonuclease H surface enzyme kinetics by surface plasmon resonance imaging and surface plasmon fluorescence spectroscopy. , 2005, Analytical chemistry.

[7]  Jihoon Park,et al.  Role of Substrate Metal in Gold Nanoparticle Enhanced Surface Plasmon Resonance Imaging , 2001 .

[8]  Danfeng Yao,et al.  Surface plasmon field-enhanced fluorescence spectroscopy studies of the interaction between an antibody and its surface-coupled antigen. , 2003, Analytical chemistry.

[9]  Keiko Tawa,et al.  Substrate-supported phospholipid membranes studied by surface plasmon resonance and surface plasmon fluorescence spectroscopy. , 2005, Biophysical journal.

[10]  Wolfgang Knoll,et al.  Parallel multispot detection of target hybridization to surface-bound probe oligonucleotides of different base mismatch by surface-plasmon field-enhanced fluorescence microscopy , 2003 .

[11]  W. Knoll,et al.  Interfaces and thin films as seen by bound electromagnetic waves. , 1998, Annual review of physical chemistry.

[12]  Donghyun Kim,et al.  Design study of highly sensitive nanowire-enhanced surface plasmon resonance biosensors using rigorous coupled wave analysis. , 2005, Optics express.

[13]  Chi-Hung Lin,et al.  Enhanced live cell membrane imaging using surface plasmon-enhanced total internal reflection fluorescence microscopy. , 2006, Optics express.

[14]  Wolfgang Knoll,et al.  Mismatching base-pair dependence of the kinetics of DNA-DNA hybridization studied by surface plasmon fluorescence spectroscopy. , 2004, Nucleic acids research.

[15]  Wolfgang Knoll,et al.  Evanescent field in surface plasmon resonance and surface plasmon field-enhanced fluorescence spectroscopies. , 2004, Analytical chemistry.