Antimicrobial resistance profi le of Vibrio species isolated from the hatchery system of Macrobrachium rosenbergii (Deman)

Vibrio spp. are responsible for high mortality and severe economic loss in prawn industry in all the prawn producing countries. The incidence of high antibiotic resistance is reported in hatcheries. For the present study, 23 isolates of Vibrio species from the larvae, larval rearing water, Artemia and Artemia rearing water from the hatchery system of Macrobrachium rosenbergii were used. The isolates were characterised and nine different species were identifi ed viz., V. harveyi, V. parahaemolyticus, V. damsela, V. splendidus, V. anguillarum, V. alginolyticus, V. fl uvialis, V. vulnificus and V. campbelli. Fifteen commonly used antibiotics in the hatchery system were tested against the isolated Vibrio species. The results showed that all the isolates (100%) were resistant to ampicillin, erythromycin, doxycycline hydrochloride and sensitive to amikacin, chloramphenicol, cefaclor, gentamycin and streptomycin.

[1]  Carly L. Daniels,et al.  Effect of dietary Bacillus spp. and mannan oligosaccharides (MOS) on European lobster (Homarus gammarus L.) larvae growth performance, gut morphology and gut microbiota , 2010 .

[2]  P. Srinivasan,et al.  Occurrence, Distribution and Antibiotic Resistance Patterns of Vibrio species associated with viral diseased Shrimp of South Indian Aquaculture Environment , 2009 .

[3]  Seong Wei Lee,et al.  Multiple Antibiotic Resistance and Heavy Metal Resistance Profile of Bacteria Isolated from Giant Freshwater Prawn （Macrobrachium rosenbergii） Hatchery , 2009 .

[4]  D. Montēt,et al.  Antibiotic resistance in Gram-negative bacteria isolated from farmed catfish , 2007 .

[5]  C. Miranda,et al.  Occurrence of florfenicol resistance in bacteria associated with two Chilean salmon farms with different history of antibacterial usage , 2007 .

[6]  Yaqing Chang,et al.  Molecular characterizations of oxytetracycline resistant bacteria and their resistance genes from mariculture waters of China. , 2006, Marine pollution bulletin.

[7]  A. Roque,et al.  Bacterial load reduction of live prey for fish larval feeding using Ox‐Aquaculture© , 2006 .

[8]  Chul Hee Choi,et al.  Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans and animals in Korea. , 2005, The Journal of antimicrobial chemotherapy.

[9]  P. Serrano.  Responsible use of antibiotics in aquaculture , 2005 .

[10]  T. J. Abraham,et al.  Distribution of luminous bacteria in semi-intensive penaeid shrimp hatcheries of Tamil Nadu, India , 2004 .

[11]  I. Salvesen,et al.  Surface disinfection of eggs from marine fish: evaluation of four chemicals , 1995, Aquaculture International.

[12]  T. J. Abraham,et al.  Antibiotic susceptibility of luminous bacteria from shrimp farm environs of West Bengal , 2003 .

[13]  A. S. S. Hameed,et al.  Antibiotic resistance in bacteria isolated from hatchery-reared larvae and post-larvae of Macrobrachium rosenbergii , 2003 .

[14]  E. A. Tendencia,et al.  Level and percentage recovery of resistance to oxytetracycline and oxolinic acid of bacteria from shrimp ponds , 2002 .

[15]  J. Teo,et al.  Genetic Determinants of Tetracycline Resistance in Vibrio harveyi , 2002, Antimicrobial Agents and Chemotherapy.

[16]  T. Nakai,et al.  Bacteriophage therapy of infectious diseases in aquaculture. , 2002, Research in microbiology.

[17]  B. Gómez‐Gil,et al.  In vitro susceptibility to 15 antibiotics of vibrios isolated from penaeid shrimps in Northwestern Mexico. , 2001, International journal of antimicrobial agents.

[18]  E. A. Tendencia,et al.  Antibiotic resistance of bacteria from shrimp ponds , 2001 .

[19]  M. L. Lizárraga-Partida,et al.  Bacteria isolated on TCBS media associated with hatched Artemia cysts of commercial brands , 2001 .

[20]  N. Kautsky,et al.  Ecosystem perspectives on management of disease in shrimp pond farming , 2000 .

[21]  T. Nakai,et al.  Isolation of Bacteriophages Specific to a Fish Pathogen, Pseudomonas plecoglossicida, as a Candidate for Disease Control , 2000, Applied and Environmental Microbiology.

[22]  A. S. S. Hameed,et al.  Antibiotic resistance in bacteria isolated from Artemia nauplii and efficacy of formaldehyde to control bacterial load , 2000 .

[23]  S. Chandrasekaran,et al.  Transfer and Expression of a Multiple Antibiotic Resistance Plasmid in Marine Bacteria , 1998, Current Microbiology.

[24]  P. Menasveta,et al.  Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth , 1998 .

[25]  D. Moriarty.  Control of luminous Vibrio species in penaeid aquaculture ponds , 1998 .

[26]  V. Inglis,et al.  Resistance to antibiotics of Vibrio spp. and Aeromonas spp. isolated from fish and shrimp tissues and rearing water in Panay island, Philippines , 1998 .

[27]  T. J. Abraham,et al.  Epibiotic infestation of luminous bacteria in penaeid shrimp Penaeus indicus (H Milne Edwards) larvae , 1997 .

[28]  P. D. Munro,et al.  Comparison of the Growth and Survival of Larval Turbot in the Absence of Culturable Bacteria with Those in the Presence of Vibrio anguillarum, Vibrio alginolyticus, or a Marine Aeromonas sp , 1995, Applied and environmental microbiology.

[29]  I. Karunasagar,et al.  Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio harveyi infection , 1994 .

[30]  Anicet R. Blanch,et al.  Improvement and update of a set of keys for biochemical identification of Vibrio species , 1994 .

[31]  A. S. S. Hameed,et al.  Studies on the chemical control of a Vibrio campbellii-like bacterium affecting hatchery-reared Penaeus indicus larvae , 1994 .

[32]  L. Albright,et al.  Studies on the sources of luminescent Vibrio harveyi in Penaeus monodon hatcheries. , 1992 .

[33]  J. O. Paclibare,et al.  The use of chemotherapeutic agents in aquaculture in the Philippines. , 1992 .

[34]  L. Ruangpan,et al.  Vibrio bacteria isolated from black tiger shrimp, Penaeus monodon Fabricius , 1991 .

[35]  K. Ikura,et al.  Ecological cycle of thermostable direct hemolysin-producing strains of Vibrio parahaemolyticus in a brackish-water area with special reference to molluscs and attached microalgae. , 1991, The Journal of veterinary medical science.

[36]  J. Swings,et al.  The microbial environment of rotifer (Brachionus plicatilis) and Artemia production systems , 1991 .

[37]  L. D. Peña,et al.  Occurrence of luminous bacterial disease of Penaeus monodon larvae in the Philippines , 1990 .

[38]  L. D. Peña,et al.  Studies on the chemical control of luminous bacteria Vibrio harveyi and V. splendidus isolated from diseased Penaeus monodon larvae and rearing water , 1990 .

[39]  R. Colwell,et al.  Characterization and distribution of Vibrio alginolyticus and Vibrio parahaemolyticus isolated in Indonesia , 1985, Applied and environmental microbiology.

[40]  C. Nash.  Giant prawn farming: michael b. new (editor). elsevier science publishers b.v., amsterdam and new york, 1982. developments in aquaculture and fisheries science, vol. 10. xii + 514 pp., us$ 97.75/dfl. 210.00, isbn 0-444-42093-2 , 1984 .