Second-messenger responses associated with stimulation of neuronal muscarinic receptors expressed by a human neuroblastoma SH-SY5Y.

Publisher Summary This chapter discusses the second-messenger responses associated with stimulation of neuronal muscarinic receptors expressed by a human neuroblastoma SH-SYSY. Stimulation of neuronal muscarinic receptors leads to a multiplicity of intracellular events, including stimulation of phosphoinositide hydrolysis, release of intracellular stored Ca2+, modulation of Ca2+ channel activity, inhibition of cAMP production, and modulation of K+ channel activity. The SH-SY5Y cell is a useful model neuronal system in which to study second messenger responses associated with muscarinic receptors, because the cell line expresses a homogeneous m3 receptor complement that subserves phosphoinositide hydrolysis, Ca2+ release from internal stores and Ca2+ channel opening. The chapter illustrates the diversity of second messenger responses associated with muscarinic receptor subtypes. The recent availability of cells with transfected muscarinic receptors coupled with single cell imaging allow further characterization of the linkage of m1–m5 subtypes to intracellular Ca2+ homeostasis and their association with inositol polyphosphate metabolism.

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