E2F: a link between the Rb tumor suppressor protein and viral oncoproteins.

The cellular transcription factor E2F, previously identified as a component of early adenovirus transcription, has now been shown to be important in cell proliferation control. E2F appears to be a functional target for the action of the tumor suppressor protein Rb that is encoded by the retinoblastoma susceptibility gene. The disruption of this E2F-Rb interaction, as well as a complex involving E2F in association with the cell cycle-regulated cyclin A-cdk2 kinase complex, may be a common mechanism of action for the oncoproteins encoded by the DNA tumor viruses.

[1]  Stephen H. Friend,et al.  Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product , 1988, Nature.

[2]  K. Münger,et al.  The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[3]  Y. Qian,et al.  The retinoblastoma gene product regulates progression through the G1 phase of the cell cycle , 1991, Cell.

[4]  R. Pepperkok,et al.  Cyclin A is required at two points in the human cell cycle. , 1992, The EMBO journal.

[5]  M. Ewen,et al.  The transcription factor E2F interacts with the retinoblastoma product and a p107-cyclin A complex in a cell cycle-regulated manner , 1992, Cell.

[6]  E. Harlow,et al.  Cellular targets for transformation by the adenovirus E1A proteins , 1989, Cell.

[7]  Anne Fernandez,et al.  Cyclin a is required for the onset of DNA replication in mammalian fibroblasts , 1991, Cell.

[8]  W. Benedict,et al.  The retinoblastoma gene functions as a growth and tumor suppressor in human bladder carcinoma cells. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[9]  K. Münger,et al.  The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. , 1989, Science.

[10]  W. Kaelin,et al.  The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein , 1991, Cell.

[11]  S. Dalton,et al.  Cell cycle regulation of the human cdc2 gene. , 1992, The EMBO journal.

[12]  Joseph R. Nevins,et al.  The E2F transcription factor is a cellular target for the RB protein , 1991, Cell.

[13]  R. Weinmann,et al.  The retinoblastoma protein copurifies with E2F-I, an E1A-regulated inhibitor of the transcription factor E2F , 1991, Cell.

[14]  M. Marton,et al.  The adenovirus E4 17-kilodalton protein complexes with the cellular transcription factor E2F, altering its DNA-binding properties and stimulating E1A-independent accumulation of E2 mRNA , 1990, Journal of virology.

[15]  J. Nevins,et al.  Identification of a cellular transcription factor involved in E1A trans-activation , 1986, Cell.

[16]  M. Ewen,et al.  Interaction between human cyclin A and adenovirus E1A-associated p107 protein. , 1992, Science.

[17]  L. Tsai,et al.  Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F , 1992, Nature.

[18]  E. Harlow,et al.  The retinoblastoma protein is phosphorylated during specific phases of the cell cycle , 1989, Cell.

[19]  M Lipp,et al.  E1A-dependent trans-activation of the human MYC promoter is mediated by the E2F factor. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[20]  M. Loeken,et al.  The adenovirus EIIA enhancer. Analysis of regulatory sequences and changes in binding activity of ATF and EIIF following adenovirus infection. , 1989, The Journal of biological chemistry.

[21]  D. R. Dubbs,et al.  Enhanced thymidine kinase activity following infection of green monkey kidney cells by simian adenoviruses, simian papovavirus SV40, and an adenovirus-SV40 "hybrid". , 1965, Virology.

[22]  S. Weintraub,et al.  Retinoblastoma protein switches the E2F site from positive to negative element , 1992, Nature.

[23]  J. Nevins,et al.  Genetic analysis of the adenovirus E4 6/7 trans activator: interaction with E2F and induction of a stable DNA-protein complex are critical for activity , 1991, Journal of virology.

[24]  R. Weinberg,et al.  G1/S phosphorylation of the retinoblastoma protein is associated with an altered affinity for the nuclear compartment , 1991, Cell.

[25]  N. L. Thangue,et al.  Adenovirus E1a prevents the retinoblastoma gene product from complexing with a cellular transcription factor , 1991, Nature.

[26]  D. Chalker,et al.  Ty3 integrates within the region of RNA polymerase III transcription initiation. , 1992, Genes & development.

[27]  J. Tooze,et al.  DNA tumor viruses , 1982 .

[28]  J. Newport,et al.  Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes , 1991, Cell.

[29]  J. Nevins,et al.  Analysis of trans activation by human papillomavirus type 16 E7 and adenovirus 12S E1A suggests a common mechanism , 1991, Journal of virology.

[30]  F. Alt,et al.  Structure and expression of the murine N-myc gene. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[31]  J. Nevins.  Mechanism of activation of early viral transcription by the adenovirus E1A gene product , 1981, Cell.

[32]  J. Nevins,et al.  Adenovirus E1A proteins can dissociate heteromeric complexes involving the E2F transcription factor: A novel mechanism for E1A trans-activation , 1990, Cell.

[33]  A. Berk.  Adenovirus promoters and E1A transactivation. , 1986, Annual review of genetics.

[34]  Pearl S Huang,et al.  Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product , 1991, Nature.

[35]  P. Branton,et al.  Detection of cellular proteins associated with human adenovirus type 5 early region 1A polypeptides. , 1985, Virology.

[36]  M. Meyerson,et al.  Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase , 1991, Nature.

[37]  A. S. Lee,et al.  Identification of a 70-base-pair cell cycle regulatory unit within the promoter of the human thymidine kinase gene and its interaction with cellular factors , 1991, Molecular and cellular biology.

[38]  M. Ewen,et al.  Interaction of p107 with cyclin A independent of complex formation with viral oncoproteins. , 1992, Science.

[39]  J. Maller,et al.  Role for cyclin A in the dependence of mitosis on completion of DMA replication , 1991, Nature.

[40]  J. Nevins,et al.  An adenovirus E4 gene product trans-activates E2 transcription and stimulates stable E2F binding through a direct association with E2F. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[41]  T. Shenk,et al.  An adenovirus early region 4 gene product is required for induction of the infection-specific form of cellular E2F activity. , 1989, Genes & development.

[42]  J. Nevins,et al.  The adenovirus E4 gene, in addition to the E1A gene, is important for trans-activation of E2 transcription and for E2F activation , 1989, Journal of virology.

[43]  J. Nevins,et al.  A cyclin A-protein kinase complex possesses sequence-specific DNA binding activity: p33cdk2 is a component of the E2F-cyclin A complex , 1992, Cell.

[44]  T. Hunt,et al.  Cyclin A and the retinoblastoma gene product complex with a common transcription factor , 1991, Nature.

[45]  N. Jones,et al.  An adenovirus type 5 early gene function regulates expression of other early viral genes. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[46]  J. Nevins,et al.  Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[47]  Marc Vidal,et al.  A cDNA encoding a pRB-binding protein with properties of the transcription factor E2F , 1992, Cell.

[48]  P. Hearing,et al.  The adenovirus early region 4 open reading frame 6/7 protein regulates the DNA binding activity of the cellular transcription factor, E2F, through a direct complex. , 1989, Genes & development.

[49]  J. Nevins,et al.  Domains of the adenovirus E1A protein required for oncogenic activity are also required for dissociation of E2F transcription factor complexes. , 1991, Genes & development.

[50]  W. Kaelin,et al.  Identification of a growth suppression domain within the retinoblastoma gene product. , 1992, Genes & development.

[51]  F. Kaye,et al.  Identification of cellular proteins that can interact specifically with the T/ElA-binding region of the retinoblastoma gene product , 1991, Cell.

[52]  Tony Hunter,et al.  Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B , 1990, Nature.

[53]  M. Ewen,et al.  Molecular cloning, chromosomal mapping, and expression of the cDNA for p107, a retinoblastoma gene product-related protein , 1991, Cell.

[54]  H. Nasheuer,et al.  Human DNA polymerase alpha gene: sequences controlling expression in cycling and serum-stimulated cells , 1991, Molecular and cellular biology.

[55]  R. Weinberg,et al.  Tumor suppressor genes. , 1991, Science.

[56]  D. R. Dubbs,et al.  DEOXYTHYMIDYLATE SYNTHETASE AND DEOXYTHYMIDINE KINASE ACTIVITIES OF VIRUS-INFECTED ANIMAL CELLS. , 1965, Cancer research.

[57]  M Lipp,et al.  Nuclear factor E2F mediates basic transcription and trans-activation by E1a of the human MYC promoter. , 1989, Genes & development.

[58]  P. Farnham,et al.  The HIP1 binding site is required for growth regulation of the dihydrofolate reductase gene promoter , 1992, Molecular and cellular biology.

[59]  J. Nevins,et al.  Activation of the E2F transcription factor in adenovirus-infected cells involves E1A-dependent stimulation of DNA-binding activity and induction of cooperative binding mediated by an E4 gene product , 1990, Journal of virology.

[60]  Phang-lang Chen,et al.  Phosphorylation of the retinoblastoma gene product is modulated during the cell cycle and cellular differentiation , 1989, Cell.

[61]  P. L. Chen,et al.  Suppression of the neoplastic phenotype by replacement of the RB gene in human cancer cells. , 1988, Science.

[62]  A. Houweling,et al.  Partial transformation of primary rat cells by the leftmost 4.5% fragment of adenovirus 5 DNA. , 1980, Virology.

[63]  Peggy J. Farnham,et al.  Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties , 1992, Cell.

[64]  J. Nevins,et al.  Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A , 1991, Cell.

[65]  J. Flint,et al.  Transcriptional and transforming activities of the adenovirus E1A proteins. , 1991, Advances in cancer research.

[66]  A. Rustgi,et al.  Amino-terminal domains of c-myc and N-myc proteins mediate binding to the retinoblastoma gene product , 1991, Nature.

[67]  J. Nevins,et al.  Role of E2F transcription factor in E1A-mediated trans activation of cellular genes , 1991, Journal of virology.

[68]  Wen-Hwa Lee,et al.  SV40 large tumor antigen forms a specific complex with the product of the retinoblastoma susceptibility gene , 1988, Cell.

[69]  J. Nevins,et al.  Role of an adenovirus E2 promoter binding factor in E1A-mediated coordinate gene control. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[70]  P. Sharp,et al.  Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs , 1979, Cell.

[71]  E. Y. Lee,et al.  A cellular protein that competes with SV40 T antigen for binding to the retinoblastoma gene product , 1991, Nature.

[72]  D. Kimelman,et al.  E1a regions of the human adenoviruses and of the highly oncogenic simian adenovirus 7 are closely related , 1985, Journal of virology.

[73]  W. Fiers,et al.  Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and simian virus 40. , 1975, Cold Spring Harbor symposia on quantitative biology.

[74]  W. Lee,et al.  Suppression of tumorigenicity of human prostate carcinoma cells by replacing a mutated RB gene. , 1990, Science.

[75]  N. Ledinko.  Stimulation of DNA synthesis and thymidine kinase activity in human embryonic kidney cells infected by Adenovirus 2 or 12. , 1967, Cancer research.