Low uptakes of Cd, Cu, and Zn in Dugesia japonica, a freshwater planarian with higher tolerance to metals

ABSTRACT Although a freshwater planarian is proposed as a potential model for studying toxicities of environmental pollutants, they are less sensitive to metal exposures, compared to other freshwater invertebrates. We hypothesised that the metal uptake is low in treated planarians, which may lead to their higher tolerance to metals. The acute toxicities of Cu and Zn to Dugesia japonica were determined. The 24- and 48-h median lethal concentrations were 4.50 and 3.46 mg L−1, respectively, for Cu, and 41.97 and 37.79 mg L−1, respectively, for Zn. Accordingly, combining with the data on Cd in our previous study, higher tolerance of planarians to metals was revealed. Also, tissue levels of Cd, Cu, and Zn in treated D. japonica were determined, and their dissolved uptake rate constants (k1) were calculated. Results showed that bioaccumulations of these metals in D. japonica were, indeed, lower than that in other freshwater invertebrates having higher susceptibilities to these metals.

[1]  Bosheng Zhao,et al.  Mortality and antioxidant responses in the planarian (Dugesia japonica) after exposure to copper , 2014, Toxicology and industrial health.

[2]  M. Galar-Martínez,et al.  Genotoxicity and oxidative stress induced by cadmium and zinc in the planarian , Dugesia dorotocephala , 2013 .

[3]  Mei-Hui Li,et al.  Bioaccumulation and Toxicodynamics of Cadmium to Freshwater Planarian and the Protective Effect of N-Acetylcysteine , 2012, Archives of Environmental Contamination and Toxicology.

[4]  Mei-Hui Li.  Survival, mobility, and membrane‐bound enzyme activities of freshwater planarian, Dugesia japonica, exposed to synthetic and natural surfactants , 2012, Environmental toxicology and chemistry.

[5]  M. Plusquin,et al.  Physiological and molecular characterisation of cadmium stress in Schmidtea mediterranea. , 2012, The International journal of developmental biology.

[6]  P. Usseglio-Polatera,et al.  Comparison of arsenate and cadmium toxicity in a freshwater amphipod (Gammarus pulex). , 2012, Environmental pollution.

[7]  Mei-Hui Li,et al.  The preferential accumulation of cadmium in the head portion of the freshwater planarian, Dugesia japonica (Platyhelminthes: Turbellaria). , 2011, Metallomics : integrated biometal science.

[8]  M. Shuhaimi-Othman,et al.  Sensitivity of the freshwater prawn, Macrobrachium lanchesteri (Crustacea: Decapoda), to heavy metals , 2011, Toxicology and industrial health.

[9]  Z. Xiufang,et al.  TOXICITY AND BEHAVIORAL EFFECTS OF CADMIUM IN PLANARIAN (Dugesia japonica Ichikawa et Kawakatsu) , 2010 .

[10]  Wen-Xiong Wang,et al.  Comparative approaches to understand metal bioaccumulation in aquatic animals. , 2008, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[11]  Wen-Xiong Wang,et al.  The influences of ambient and body calcium on cadmium and zinc accumulation in Daphnia magna , 2008, Environmental toxicology and chemistry.

[12]  Theodore Garland,et al.  Aquatic insect ecophysiological traits reveal phylogenetically based differences in dissolved cadmium susceptibility , 2008, Proceedings of the National Academy of Sciences.

[13]  H. Ferreira,et al.  Evaluation of copper effects upon Girardia tigrina freshwater planarians based on a set of biomarkers. , 2008, Chemosphere.

[14]  Mei-Hui Li.  Effects of nonionic and ionic surfactants on survival, oxidative stress, and cholinesterase activity of planarian. , 2008, Chemosphere.

[15]  S. Luoma,et al.  Cadmium biodynamics in the oligochaete Lumbriculus variegatus and its implications for trophic transfer. , 2008, Aquatic toxicology.

[16]  S. Adhikari,et al.  Bioaccumulation of copper in post-larvae and juveniles of freshwater prawn Macrobrachium rosenbergii (de Man) exposed to sub-lethal levels of copper sulfate , 2006 .

[17]  G. Kovačević,et al.  Toxicity testing of herbicide norflurazon on an aquatic bioindicator species--the planarian Polycelis felina (Daly.). , 2005, Aquatic toxicology.

[18]  Jui-Pin Wu,et al.  Metallothionein induction and heavy metal accumulation in white shrimp Litopenaeus vannamei exposed to cadmium and zinc. , 2005, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[19]  Colin R. Janssen,et al.  Effects of chronic dietary copper exposure on growth and reproduction of Daphnia magna , 2004, Environmental toxicology and chemistry.

[20]  Zeng-Hui Zhao,et al.  Acute toxicity of Cu~(2+), Hg~(2+) and Pb~(2+) TO PLANARIAN (Dugesia japonica) , 2004 .

[21]  M. S. Benfato,et al.  Stress protein response and catalase activity in freshwater planarian Dugesia (Girardia) schubarti exposed to copper. , 2003, Ecotoxicology and environmental safety.

[22]  A. Hendriks,et al.  Temperature-dependent effects of cadmium on Daphnia magna: accumulation versus sensitivity. , 2003, Environmental science & technology.

[23]  J. Henriques,et al.  Genotoxic effects of copper sulphate in freshwater planarian in vivo, studied with the single-cell gel test (comet assay). , 2001, Mutation research.

[24]  F. Pontieri,et al.  Acetylcholine/dopamine interaction in planaria. , 2000, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP.

[25]  N. V. van Straalen,et al.  Combining life-history and toxicokinetic parameters to interpret differences in sensitivity to cadmium between populations of Polycelis tenuis (Platyhelminthes). , 1999, Ecotoxicology and environmental safety.

[26]  K. Güven,et al.  Acute Lethal Toxicity and Accumulation of Copper in Gammarus pulex (L.) (Amphipoda) , 1999 .

[27]  F. Calevro,et al.  Toxic effects of aluminium, chromium and cadmium in intact and regenerating freshwater planarians , 1998 .

[28]  F. Stocchi,et al.  A pharmacological study of cocaine activity in planaria. , 1996, Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology.

[29]  C. Mouvet,et al.  Laboratory accumulation and depuration of copper and cadmium in the freshwater mussel Dreissena polymorpha and the aquatic moss Rhynchostegium riparioides , 1993 .

[30]  D. Schaeffer,et al.  Planarians in toxicology. Responses of asexualDugesia dorotocephala to selected metals , 1991, Bulletin of environmental contamination and toxicology.

[31]  D. Schaeffer,et al.  Neurobehavioral toxicity of cadmium sulfate to the planarianDugesia dorotocephala , 1991, Bulletin of environmental contamination and toxicology.

[32]  F. Stocchi,et al.  A pharmacological study of dopaminergic receptors in planaria , 1989, Neuropharmacology.

[33]  D. Wright,et al.  Effect of calcium on cadmium uptake and toxicity in larvae and juveniles of striped bass (Morone saxatilis) , 1985, Bulletin of environmental contamination and toxicology.

[34]  D. Wright,et al.  The effect of calcium on cadmium toxicity in the freshwater amphipod,Gammarus pulex (L.) , 1981, Archives of environmental contamination and toxicology.

[35]  B. Bayne.  Ventilation, the heart beat and oxygen uptake by Mytilus edulis L. in declining oxygen tension , 1971 .