RepPop: a database for repetitive elements in Populus trichocarpa

BackgroundPopulus trichocarpa is the first tree genome to be completed, and its whole genome is currently being assembled. No functional annotation about the repetitive elements in the Populus trichocarpa genome is currently available.ResultsWe predicted 9,623 repetitive elements in the Populus trichocarpa genome, and assigned functions to 3,075 of them (31.95%). The 9,623 repetitive elements cover ~40% of the current (partially) assembled genome. Among the 9,623 repetitive elements, 668 have copies only in the contigs that have not been assigned to one of the 19 chromosome while the rest all have copies in the partially assembled chromosomes.ConclusionAll the predicted data are organized into an easy-to-use web-browsable database, RepPop. Various search capabilities are provided against the RepPop database. A Wiki system has been set up to facilitate functional annotation and curation of the repetitive elements by a community rather than just the database developer. The database RepPop will facilitate the assembling and functional characterization of the Populus trichocarpa genome.

[1]  G. Benson,et al.  Tandem repeats finder: a program to analyze DNA sequences. , 1999, Nucleic acids research.

[2]  M. Ayub,et al.  Production of ethanol from soybean hull hydrolysate by osmotolerant Candida guilliermondii NRRL Y-2075. , 2008, Bioresource technology.

[3]  G. Taylor,et al.  Populus: arabidopsis for forestry. Do we need a model tree? , 2002, Annals of botany.

[4]  H. Saedler,et al.  IS2, a genetic element for turn-off and turn-on of gene activity in E. coli , 2004, Molecular and General Genetics MGG.

[5]  Victor G. Levitsky,et al.  RECON: a program for prediction of nucleosome formation potential , 2004, Nucleic Acids Res..

[6]  Wolf-Dietrich Heyer,et al.  Homologous recombination in DNA repair and DNA damage tolerance , 2008, Cell Research.

[7]  M. Gribskov,et al.  The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray) , 2006, Science.

[8]  J. Jurka,et al.  Repbase Update, a database of eukaryotic repetitive elements , 2005, Cytogenetic and Genome Research.

[9]  G. Tuskan,et al.  Characterization of microsatellites revealed by genomic sequencing of Populus trichocarpa , 2004 .

[10]  R. Lenski,et al.  Long-term experimental evolution in Escherichia coli. IX. Characterization of insertion sequence-mediated mutations and rearrangements. , 2000, Genetics.

[11]  Jing-Ke Weng,et al.  Improvement of biomass through lignin modification. , 2008, The Plant journal : for cell and molecular biology.

[12]  James R. Cole,et al.  The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data , 2006, Nucleic Acids Res..

[13]  J. Wootton,et al.  Analysis of compositionally biased regions in sequence databases. , 1996, Methods in enzymology.

[14]  The Arabidopsis Genome Initiative Analysis of the genome sequence of the flowering plant Arabidopsis thaliana , 2000, Nature.

[15]  Takuji Sasaki,et al.  The map-based sequence of the rice genome , 2005, Nature.

[16]  Pavel A. Pevzner,et al.  De novo identification of repeat families in large genomes , 2005, ISMB.

[17]  A. Wright,et al.  Insertion of DNA activates the cryptic bgl operon in E. coli K12 , 1981, Nature.

[18]  Eugene W. Myers,et al.  PILER : identification and classification of genomic repeats , 2005 .

[19]  Andrea Zuccolo,et al.  RetrOryza: a database of the rice LTR-retrotransposons , 2006, Nucleic Acids Res..

[20]  M. L. Lopes,et al.  Yeast selection for fuel ethanol production in Brazil. , 2008, FEMS yeast research.

[21]  H. Saedler,et al.  IS1 is involved in deletion formation in the gal region of E. coli K12 , 2004, Molecular and General Genetics MGG.

[22]  J. Roth,et al.  Transposon Tn10 provides a promoter for transcription of adjacent sequences. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[23]  Gregory D. Schuler,et al.  Database resources of the National Center for Biotechnology Information , 2021, Nucleic Acids Res..

[24]  J. Bennetzen,et al.  A unified classification system for eukaryotic transposable elements , 2007, Nature Reviews Genetics.

[25]  Sean R. Eddy,et al.  Rfam: annotating non-coding RNAs in complete genomes , 2004, Nucleic Acids Res..

[26]  R. Flavell,et al.  Characterisation of the wheat genome by renaturation kinetics , 1975, Chromosoma.

[27]  Yoshihiro Kawahara,et al.  The Rice Annotation Project Database (RAP-DB): 2008 update , 2007, Nucleic Acids Res..

[28]  S. Eddy,et al.  tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. , 1997, Nucleic acids research.

[29]  J. Bouché,et al.  Characterization and properties of very large inversions of the E. coli chromosome along the origin-to-terminus axis , 2004, Molecular and General Genetics MGG.