This paper presents a massively multiplexed and quantitative immunohistochemistry (IHC) platform using microfluidics technology. The device was fabricated with two-step multiplayer soft lithography and a microvalve was integrated to simultaneously flow 10 biomarkers into individual reaction channel. The microfluidic massive IHC system enabled 20 IHC assays on a biological specimen at the same time, saving 16-fold time consumption. Moreover, the expressions of the biomarkers were quantitatively compared. The immunohistochemical staining from the microfluidic system showed accurate localization and comparable quality with that of conventional IHC method. Proteomic profiling was examined for 4 breast cancer cell lines, AU-565, HCC70, MCF-7 and SK-BR-3. The microfluidic massive IHC system is expected to be useful for accurate histopathological diagnosis using numerous specific biomarkers at a time.
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