Magnetic nanoparticle-enhanced biosensor based on grating-coupled surface plasmon resonance.

A highly sensitive surface plasmon resonance (SPR) biosensor employing magnetic nanoparticle (MNP) assays is presented. In the reported approach, MNPs simultaneously served as "vehicles" for rapid delivery of target analyte from a sample to the sensor surface and as labels increasing the measured refractive index changes that are associated with the binding of target analyte. An optical setup based on grating-coupled surface plasmon resonance (GC-SPR) was used with a magnetic field gradient applied through the sensor chip for manipulating with MNPs on its surface. Iron oxide MNPs and a sensor surface with metallic diffraction grating were modified with antibodies that specifically recognize different epitopes of the analyte of interest. The sensitivity of the biosensor was investigated as a function of mass transport of the analyte to the sensor surface driven by diffusion (free analyte) or by the magnetic field gradient (analyte bound to MNPs). Immunoassay-based detection of β human chorionic gonadotropin (βhCG) was implemented to evaluate the sensitivity of the MNP-enhanced GC-SPR biosensor scheme. The results reveal that the sensitivity of βhCG detection was improved by 4 orders of magnitude compared with the regular SPR sensor with direct detection format, and a limit of detection below pM was achieved.

[1]  Yong-Kweon Kim,et al.  Multifunctional silver-embedded magnetic nanoparticles as SERS nanoprobes and their applications. , 2010, Small.

[2]  Nicole Pamme,et al.  Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow. , 2009, Lab on a chip.

[3]  Yusuke Arima,et al.  Surface plasmon resonance-based highly sensitive immunosensing for brain natriuretic peptide using nanobeads for signal amplification. , 2006, Analytical biochemistry.

[4]  C. Mirkin,et al.  Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins , 2003, Science.

[5]  J. Homola.  Present and future of surface plasmon resonance biosensors , 2003, Analytical and bioanalytical chemistry.

[6]  Bing Xu,et al.  Using biofunctional magnetic nanoparticles to capture vancomycin-resistant enterococci and other gram-positive bacteria at ultralow concentration. , 2003, Journal of the American Chemical Society.

[7]  Lin He,et al.  Colloidal Au-Enhanced Surface Plasmon Resonance for Ultrasensitive Detection of DNA Hybridization , 2000 .

[8]  W. Knoll,et al.  Surface plasmon fluorescence measurements of human chorionic gonadotrophin: role of antibody orientation in obtaining enhanced sensitivity and limit of detection. , 2005, Analytical chemistry.

[9]  Ralph Weissleder,et al.  Peroxidase Substrate Nanosensors for MR Imaging , 2004 .

[10]  Shiping Fang,et al.  Attomole microarray detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions. , 2006, Journal of the American Chemical Society.

[11]  Itamar Willner,et al.  Magnetic control of electrocatalytic and bioelectrocatalytic processes. , 2003, Angewandte Chemie.

[12]  J. Homola.  Surface plasmon resonance sensors for detection of chemical and biological species. , 2008, Chemical reviews.

[13]  Cristina Tortolini,et al.  Multifunctional au nanoparticle dendrimer-based surface plasmon resonance biosensor and its application for improved insulin detection. , 2010, Analytical chemistry.

[14]  Guannan Wang,et al.  Multiplex immunoassays of equine virus based on fluorescent encoded magnetic composite nanoparticles , 2010, Analytical and bioanalytical chemistry.

[15]  Zanzan Zhu,et al.  Magnetic nanoparticle enhanced surface plasmon resonance sensing and its application for the ultrasensitive detection of magnetic nanoparticle-enriched small molecules. , 2010, Analytical chemistry.

[16]  Abraham J. Qavi,et al.  Nanoparticle-enhanced diffraction gratings for ultrasensitive surface plasmon biosensing. , 2007, Analytical chemistry.

[17]  I-Ming Hsing,et al.  Micro‐ and Nano‐ Magnetic Particles for Applications in Biosensing , 2007 .

[18]  H. Tsai,et al.  Determination of hepatitis B surface antigen using magnetic immunoassays in a thin channel. , 2010, Biosensors & bioelectronics.

[19]  B Goldstein,et al.  Transport effects on surface–volume biological reactions , 1999, Journal of mathematical biology.

[20]  Chi‐Huey Wong,et al.  Iron oxide/gold core/shell nanoparticles for ultrasensitive detection of carbohydrate-protein interactions. , 2009, Analytical chemistry.

[21]  R. Corn,et al.  Enhanced bioaffinity sensing using surface plasmons, surface enzyme reactions, nanoparticles and diffraction gratings. , 2008, The Analyst.

[22]  Ralph Weissleder,et al.  Magnetic relaxation switches capable of sensing molecular interactions , 2002, Nature Biotechnology.

[23]  W. Knoll,et al.  Interfaces and thin films as seen by bound electromagnetic waves. , 1998, Annual review of physical chemistry.

[24]  B. Varughese,et al.  Magnetic iron oxide nanoparticles for biorecognition: evaluation of surface coverage and activity. , 2006, The journal of physical chemistry. B.

[25]  Wolfgang Knoll,et al.  Immunosensor with self-referencing based on surface plasmon diffraction. , 2004, Analytical chemistry.

[26]  I. Willner,et al.  Magnetoswitchable reactions of DNA monolayers on electrodes: gating the processes by hydrophobic magnetic nanoparticles. , 2005, Journal of the American Chemical Society.

[27]  Moo-Yeal Lee,et al.  Gene delivery in three-dimensional cell cultures by superparamagnetic nanoparticles. , 2010, ACS nano.

[28]  Yi Wang,et al.  Prostate specific antigen biosensor based on long range surface plasmon-enhanced fluorescence spectroscopy and dextran hydrogel binding matrix. , 2009, Analytical chemistry.

[29]  R. Corn,et al.  Direct detection of genomic DNA by enzymatically amplified SPR imaging measurements of RNA microarrays. , 2004, Journal of the American Chemical Society.

[30]  R. Corn,et al.  Enzymatically amplified surface plasmon resonance imaging detection of DNA by exonuclease III digestion of DNA microarrays. , 2005, Analytical chemistry.

[31]  Dino Di Carlo,et al.  Rapid and dynamic intracellular patterning of cell-internalized magnetic fluorescent nanoparticles. , 2009, Nano letters.

[32]  Jiří Homola,et al.  Surface plasmon resonance sensor based on an array of diffraction gratings for highly parallelized observation of biomolecular interactions , 2008 .

[33]  Mwj Menno Prins,et al.  Rapid integrated biosensor for multiplexed immunoassays based on actuated magnetic nanoparticles. , 2009, Lab on a chip.

[34]  J. Mitchell,et al.  Sensitivity enhancement of surface plasmon resonance biosensing of small molecules. , 2005, Analytical biochemistry.

[35]  A. Limaye,et al.  Surface plasmon resonance detection using antibody-linked magnetic nanoparticles for analyte capture, purification, concentration, and signal amplification. , 2009, Analytical chemistry.

[36]  Jiří Homola,et al.  Surface plasmon resonance sensors based on diffraction gratings and prism couplers: sensitivity comparison , 1999 .

[37]  Jiří Homola,et al.  Surface functionalization for self-referencing surface plasmon resonance (SPR) biosensors by multi-step self-assembly , 2003 .

[38]  Marco H Hefti,et al.  Rapid, high sensitivity, point-of-care test for cardiac troponin based on optomagnetic biosensor. , 2010, Clinica chimica acta; international journal of clinical chemistry.

[39]  Nan Zhang,et al.  A disposable polymer sensor chip combined with micro-fluidics and surface plasmon read-out. , 2009, Biosensors & bioelectronics.