GLYCOSYLTRANSFERASES OF RAT BRAIN THAT MAKE CEREBROSIDES: SUBSTRATE SPECIFICITY, INHIBITORS, AND ABNORMAL PRODUCTS 1

Brain homogenates from young rats were assayed for their ability to synthesize cerebrosides from radioactive UDP‐galactose or UDP‐glucose and ceramide. A comparison of galactose transfer with ceramides made from different 2‐hydroxy acids showed that the shortest one tested (C7) was by far the best acceptor, while the poorest contained 18 carbon atoms; longer fatty acids were better than CIS. Glucosyltransferase, on the other hand, showed rather little chain length specificity or discrimination against hydroxy acid ceramides.

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