The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody.

[1]  N J Stark,et al.  Glucose-dependent glycosylation of secretory glycoprotein in mouse myeloma cells. , 1979, Archives of biochemistry and biophysics.

[2]  P. Parren,et al.  Mutation of Y407 in the CH3 domain dramatically alters glycosylation and structure of human IgG , 2013, mAbs.

[3]  P. Taylor,et al.  Anti-inflammatory activity of IgG1 mediated by Fc galactosylation and association of FcγRIIB and dectin-1 , 2012, Nature Medicine.

[4]  R. Werner,et al.  Glycosylation of therapeutic proteins in different production systems , 2007, Acta paediatrica.

[5]  Peifeng Chen,et al.  Differential display identifies genes in chinese hamster ovary cells sensitive to elevated ammonium , 2007, Applied biochemistry and biotechnology.

[6]  K. Gull,et al.  Recombinant human interferon-gamma. Differences in glycosylation and proteolytic processing lead to heterogeneity in batch culture. , 1990, The Biochemical journal.

[7]  Gregory Stephanopoulos,et al.  Metabolic effects on recombinant interferon-gamma glycosylation in continuous culture of Chinese hamster ovary cells , 2000, IBM J. Res. Dev..

[8]  Eleftherios T. Papoutsakis,et al.  Culture pH Affects Expression Rates and Glycosylation of Recombinant Mouse Placental Lactogen Proteins by Chinese Hamster Ovary (CHO) Cells , 1993, Bio/Technology.

[9]  T. Hamblin,et al.  Acquisition of potential N-glycosylation sites in the immunoglobulin variable region by somatic mutation is a distinctive feature of follicular lymphoma. , 2002, Blood.

[10]  D. Forciniti,et al.  Effect of glycosylation on the partition behavior of a human antibody in aqueous two‐phase systems , 2013, Biotechnology progress.

[11]  Markus Aebi,et al.  Oligosaccharyltransferase: the central enzyme of N-linked protein glycosylation , 2011, Journal of Inherited Metabolic Disease.

[12]  R. Parekh,et al.  Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid. , 1995, Analytical biochemistry.

[13]  Pauline M Rudd,et al.  Detailed structural analysis of N-glycans released from glycoproteins in SDS-PAGE gel bands using HPLC combined with exoglycosidase array digestions. , 2006, Methods in molecular biology.

[14]  A. R. Costa,et al.  The impact of microcarrier culture optimization on the glycosylation profile of a monoclonal antibody , 2013, SpringerPlus.

[15]  J. Deisenhofer.  Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9- and 2.8-A resolution. , 1981, Biochemistry.

[16]  H Li,et al.  Gamma-interferon production and quality in stoichiometric fed-batch cultures of Chinese hamster ovary (CHO) cells under serum-free conditions. , 1997, Biotechnology and bioengineering.

[17]  Gregory Stephanopoulos,et al.  Metabolic effects on recombinant interferon‐γ glycosylation in continuous culture of Chinese hamster ovary cells , 1999 .

[18]  Jason Dean,et al.  Metabolic analysis of antibody producing CHO cells in fed‐batch production , 2013, Biotechnology and bioengineering.

[19]  S. Kornfeld,et al.  Glucose starvation alters lipid-linked oligosaccharide biosynthesis in Chinese hamster ovary cells. , 1981, The Journal of biological chemistry.

[20]  J. Goergen,et al.  Influence of intracellular nucleotide and nucleotide sugar contents on recombinant interferon‐γ glycosylation during batch and fed‐batch cultures of CHO cells , 2008, Biotechnology and bioengineering.

[21]  M. Lehrman,et al.  Non-radioactive analysis of lipid-linked oligosaccharide compositions by fluorophore-assisted carbohydrate electrophoresis. , 2006, Methods in enzymology.

[22]  A. R. Costa,et al.  The impact of cell adaptation to serum-free conditions on the glycosylation profile of a monoclonal antibody produced by Chinese hamster ovary cells. , 2013, New biotechnology.

[23]  Roy Jefferis,et al.  Glycosylation as a strategy to improve antibody-based therapeutics , 2009, Nature Reviews Drug Discovery.

[24]  Pauline M. Rudd,et al.  GlycoBase and autoGU: tools for HPLC-based glycan analysis , 2008, Bioinform..

[25]  T Miyamoto,et al.  Effects of galactose depletion from oligosaccharide chains on immunological activities of human IgG. , 1989, The Journal of rheumatology.

[26]  R. Jefferis,et al.  Sialylation of human IgG-Fc carbohydrate by transfected rat alpha2,6-sialyltransferase. , 2001, Biochemical and biophysical research communications.

[27]  M. Butler,et al.  Optimisation of the Cellular Metabolism of Glycosylation for Recombinant Proteins Produced by Mammalian Cell Systems , 2006, Cytotechnology.

[28]  Armin Ruf,et al.  Unique carbohydrate–carbohydrate interactions are required for high affinity binding between FcγRIII and antibodies lacking core fucose , 2011, Proceedings of the National Academy of Sciences.

[29]  D. Karaoglu,et al.  Allosteric regulation provides a molecular mechanism for preferential utilization of the fully assembled dolichol-linked oligosaccharide by the yeast oligosaccharyltransferase. , 2001, Biochemistry.

[30]  Pauline M Rudd,et al.  A systematic approach to protein glycosylation analysis: a path through the maze. , 2010, Nature chemical biology.

[31]  M. Butler,et al.  Fed‐batch CHO cell t‐PA production and feed glutamine replacement to reduce ammonia production , 2013, Biotechnology progress.

[32]  M. Butler,et al.  Glycosylation of an immunoglobulin produced from a murine hybridoma cell line: The effect of culture mode and the anti‐apoptotic gene, bcl‐2 , 2007, Biotechnology and bioengineering.

[33]  Michael Butler,et al.  Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals , 2005, Applied Microbiology and Biotechnology.

[34]  B. Scallon,et al.  Higher levels of sialylated Fc glycans in immunoglobulin G molecules can adversely impact functionality. , 2007, Molecular immunology.

[35]  Chew-Kiat Heng,et al.  Impact of dynamic online fed-batch strategies on metabolism, productivity and N-glycosylation quality in CHO cell cultures. , 2005 .

[36]  Xin Liu,et al.  Transient expression and purification of chimeric heavy chain antibodies. , 2009, Protein expression and purification.

[37]  M. Butler,et al.  Dissolved oxygen concentration in serum-free continuous culture affects N-linked glycosylation of a monoclonal antibody. , 1998, Journal of biotechnology.

[38]  Royston Goodacre,et al.  Effective quenching processes for physiologically valid metabolite profiling of suspension cultured Mammalian cells. , 2009, Analytical chemistry.

[39]  Daniela Bumbaca,et al.  Physiochemical and Biochemical Factors Influencing the Pharmacokinetics of Antibody Therapeutics , 2012, The AAPS Journal.

[40]  M B Sliwkowski,et al.  Ammonium alters N-glycan structures of recombinant TNFR-IgG: degradative versus biosynthetic mechanisms. , 2000, Biotechnology and bioengineering.

[41]  J. Ravetch,et al.  Anti-Inflammatory Activity of Immunoglobulin G Resulting from Fc Sialylation , 2006, Science.

[42]  D. James,et al.  CHO cell line specific prediction and control of recombinant monoclonal antibody N‐glycosylation , 2013, Biotechnology and bioengineering.

[43]  R. Radcliffe,et al.  Sodium butyrate alters erythropoietin glycosylation via multiple mechanisms , 2008, Biotechnology and bioengineering.

[44]  A. Bull,et al.  Glucose‐limited chemostat culture of chinese hamster ovary cells producing recombinant human interferon‐γ , 1992, Biotechnology and bioengineering.

[45]  D. James,et al.  Control of Recombinant Monoclonal Antibody Effector Functions by Fc N‐Glycan Remodeling in Vitro , 2005, Biotechnology progress.

[46]  H. Broly,et al.  A high-throughput media design approach for high performance mammalian fed-batch cultures , 2013, mAbs.

[47]  R. Jefferis.  Isotype and glycoform selection for antibody therapeutics. , 2012, Archives of biochemistry and biophysics.

[48]  P. V. van Berkel,et al.  Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose , 2011, Biotechnology and bioengineering.

[49]  M. Betenbaugh,et al.  Analysis and metabolic engineering of lipid-linked oligosaccharides in glycosylation-deficient CHO cells. , 2010, Biochemical and biophysical research communications.

[50]  C. Goochee,et al.  The effect of cell-culture conditions on the oligosaccharide structures of secreted glycoproteins. , 1994, Current opinion in biotechnology.

[51]  T. Hennet,et al.  N-Glycosylation Site Occupancy in Serum Glycoproteins Using Multiple Reaction Monitoring Liquid Chromatography-Mass Spectrometry* , 2007, Molecular & Cellular Proteomics.

[52]  F. Wurm.  Production of recombinant protein therapeutics in cultivated mammalian cells , 2004, Nature Biotechnology.

[53]  R. Bayer,et al.  The impact of glycosylation on monoclonal antibody conformation and stability , 2011, mAbs.

[54]  R. Jordan,et al.  Galactosylation variations in marketed therapeutic antibodies , 2012, mAbs.

[55]  Danny Chee Furng Wong,et al.  Impact of dynamic online fed-batch strategies on metabolism, productivity and N-glycosylation quality in CHO cell cultures. , 2005, Biotechnology and bioengineering.

[56]  C. Hoy,et al.  Multiple cell culture factors can affect the glycosylation of Asn-184 in CHO-produced tissue-type plasminogen activator. , 2000, Biotechnology and bioengineering.

[57]  Daniel I. C. Wang,et al.  Improvement of interferon-gamma sialylation in Chinese hamster ovary cell culture by feeding of N-acetylmannosamine. , 1998, Biotechnology and bioengineering.