The neutrophil‐activating protein (HP‐NAP) of Helicobacter pylori is a potent stimulant of mast cells

Infection by Helicobacter pylori causes an acute inflammatory response followed by a chronic infection of the human gastric mucosa. A neutrophil‐activating protein (HP‐NAP) has been identified in H. pylori, and its role in infection and immune response is currently under investigation. Here, we show that HP‐NAP induces β‐hexosaminidase release and interleukin‐6 production in peritoneal mast cells, two actions which are completely inhibited by pertussis toxin. We also show that in polarized epithelial cell monolayers HP‐NAP translocates from the apical to thebasolateral domain, where mast cells are located. These findings characterize HP‐NAP as an inflammatory factor of H. pylori that is effective from the beginning of the inflammatory cascade.

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