Immobilization of glucose oxidase onto the blend membrane of poly(vinyl alcohol) and regenerated silk fibroin: morphology and application to glucose biosensor

Glucose oxidase was immobilized onto the blend membrane of poly(vinyl alcohol) (PVA) and regernerated silk fibroin (RSF). In order to decrease the resistance to material transport of substrate, the porous blend membranes of the immobilized enzyme were prepared by adding poly(ethylene glycol) as a reagent for making holes into the blend solution. The structures of these blend membranes were investigated with SEM. On the basis of the morphology of the blend membranes, the mechanism of forming porous blend membranes was suggested as one of phase inversion. A glucose biosensor was, for the first time, constructed by utilizing the immobilized-enzyme membrane coupling with O2 electrode. The parameters of the glucose biosensor, such as response time, stability, and effect on pH and temperature, were examined. Moreover, properties of the immobilized enzyme onto the blend membrane of PVA and RSF were compared with those of free enzyme and the immobilized enzyme onto the RSF membrane.

[1]  I. Karube,et al.  Biosensors fob food industry , 1987 .

[2]  R. Béliveau,et al.  Development of Biosensors Based on Immobilization of Enzymes in Eastman AQ Polymer Coated with a Layer of Nafion , 1990 .

[3]  J. Qian,et al.  Immobilization of glucose oxidase in the regenerated silk fibroin membrane: Characterization of the membrane structure and its application to an amperometric glucose sensor employing ferrocene as electron shuttle , 1995 .

[4]  A. M. Yacynych,et al.  Electrochemical Sensors in the Analysis and Control of Bioprocesses , 1986, Biotechnology progress.

[5]  M. Šnejdárková,et al.  Design of a glucose minisensor based on streptavidin-glucose oxidase complex coupling with self-assembled biotinylated phospholipid membrane on solid support. , 1993, Analytical chemistry.

[6]  A. Amine,et al.  Characterization of Mixed Enzyme-Mediator-Carbon Paste Electrodes , 1993 .

[7]  Joseph Wang,et al.  Miniaturized glucose sensors based on electrochemical codeposition of rhodium and glucose oxidase onto carbon-fiber electrodes , 1992 .

[8]  A. Turner,et al.  An Enzyme Electrode for Glucose Consisting of Glucose Oxidase Immobilised at a Benzoquinone-Modified Carbon Electrode , 1991 .

[9]  Claudia Danilowicz,et al.  Enzyme catalysis at hydrogel-modified electrodes with redox polymer mediator , 1993 .

[10]  C. Liu,et al.  Polymeric redox mediator enzyme electrodes for anaerobic glucose monitoring , 1993 .

[11]  A Heller,et al.  Amperometric glucose microelectrodes prepared through immobilization of glucose oxidase in redox hydrogels. , 1991, Analytical chemistry.

[12]  D J Clarke,et al.  The development and application of biosensing devices for bioreactor monitoring and control. , 1985, Biosensors.

[13]  R. Nakamura,et al.  Future of biosensors in the clinical laboratory , 1988 .

[14]  H. S. Lee,et al.  Amperometric enzyme‐modified electrodes based on tetrathiafulvalene derivatives for the determination of glucose , 1992 .

[15]  A. Turner,et al.  Glucose oxidase: an ideal enzyme , 1992 .

[16]  R. Lal,et al.  Conducting polymer-based biosensors , 1994 .

[17]  G G Guilbault,et al.  Clinical uses of enzyme electrode probes. , 1986, Biosensors.

[18]  Christopher R. Lowe,et al.  Covalent electropolymerization of glucose oxidase in polypyrrole , 1992 .

[19]  Daren J. Caruana,et al.  Electrochemical immobilization of enzymes. Part V. Microelectrodes for the detection of glucose based on glucose oxidase immobilized in a poly(phenol) film , 1992 .

[20]  Kaw Jl,et al.  Enzyme-modified organic conducting salt microelectrode , 1991 .

[21]  H. S. Lee,et al.  Electrical Communication Between Glucose Oxidase and Novel Ferrocene-Containing Siloxane-Ethylene Oxide Copolymers: Biosensor Applications , 1993 .