Challenges in the identification of species of canned fish

Abstract The identification of fish species becomes a problem when the usual identifying characteristics are removed on processing and only a portion of flesh is available. When the flesh is raw or cooked under normal conditions, the species is readily established by electrophoresis of the muscle proteins. The procedure cannot be used for heat-sterilised canned fish as the proteins are severely denatured. DNA is also degraded but techniques are now available for targeting and amplifying species-specific fragments. The amplified products can then be analysed by a range of techniques some of which are suitable for food control laboratories.

[1]  Evolution of mitochondrial DNA in fishes , 2022 .

[2]  Jeffrey L. Ram,et al.  Authentication of Canned Tuna and Bonito by Sequence and Restriction Site Analysis of Polymerase Chain Reaction Products of Mitochondrial DNA , 1996 .

[3]  H. Rehbein.  Electrophoretic techniques for species identification of fishery products , 1990 .

[4]  R. Pérez-Martín,et al.  Fish species identification in Canned Tuna by DNA Analysis (PCR-SSCP) , 1995 .

[5]  A. Meyer,et al.  Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[6]  William S. Davidson,et al.  Identification of Thunnus Tuna Species by the Polymerase Chain Reaction and Direct Sequence Analysis of their Mitochondrial Cytochrome b Genes , 1991 .

[7]  S. H. Ashoor,et al.  Liquid chromatographic identification of common fish species , 1987 .

[8]  R. Pérez-Martín,et al.  Fish species identification in canned tuna by PCR-SSCP : validation by a collaborative study and investigation of intra-species variability of the DNA-patterns , 1999 .

[9]  J. M. Gallardo,et al.  Fish species identification in seafood products , 1993 .

[10]  E. Gross.  [27] The cyanogen bromide reaction , 1967 .

[11]  W. Davidson,et al.  FINS (forensically informative nucleotide sequencing): A procedure for identifying the animal origin of biological specimens. , 1992, BioTechniques.

[12]  R. Pérez-Martín,et al.  Comparison of different methods to produce single‐strand DNA for identification of canned tuna by single‐strand conformation polymorphism analysis , 1998, Electrophoresis.

[13]  L. Sheen,et al.  Trends in Food Science , 1989 .

[14]  Ricardo I. Pérez-Martín,et al.  Use of mtDNA Direct Polymerase Chain Reaction (PCR) Sequencing and PCR-Restriction Fragment Length Polymorphism Methodologies in Species Identification of Canned Tuna , 1998 .

[15]  J. M. Gallardo,et al.  Use of capillary zone electrophoresis for fish species identification. Differentiation of flatfish species , 1995 .