Blue and yellow laccases of ligninolytic fungi.

Extracellular laccases from submerged cultures of Coriolus versicolor BKM F-116, Panus tigrinus 8/18, Phlebia radiata 79 (ATCC 64658), Phlebia tremellosa 77-51 and from cultures of Pa. tigrinus 8/18, Ph. radiata 79 and Agaricus bisporus D-649 grown on wheat straw (solid-state fermentation) were purified. All enzymes from submerged cultures had a blue colour and characteristic absorption and EPR spectra. Laccases from the solid-state cultures were yellow-brown and had no typical blue oxidase spectra and also showed atypical EPR spectra. Comparison of N-terminal amino acid sequences of purified laccases showed high homology between blue and yellow-brown laccase forms. Formation of yellow laccases as a result of binding of lignin-derived molecules by enzyme protein is proposed.

[1]  L. Golovleva,et al.  Oxidase of the white rot fungus Panus tigrinus 8/18 , 1994, FEBS letters.

[2]  D. Wood,et al.  Lignocellulose biodegradation by Agaricus bisporus during solid substrate fermentation , 1991 .

[3]  J. M. Fernández-Abalos,et al.  Purification and characterization of a phenoloxidase (laccase) from the lignin-degrading basidiomycete PM1 (CECT 2971) , 1993, Applied and environmental microbiology.

[4]  C. Merril,et al.  Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. , 1981, Science.

[5]  D. Wood.  Production, Purification and Properties of Extracellular Laccase of Agaricus bisporus , 1980 .

[6]  W. Timberlake,et al.  Sequence and molecular structure of the Aspergillus nidulans yA (laccase I) gene. , 1990, Nucleic acids research.

[7]  L. Golovleva,et al.  COMPARATIVE CHARACTERIZATION OF OXIDASE-1 FROM PANUS TIGRINUS 8-18 AND LACCASE AND FROM CORIOLUS VERSICOLOR VKM-116 , 1996 .

[8]  T. Vares,et al.  Lignin Peroxidases, Manganese Peroxidases, and Other Ligninolytic Enzymes Produced by Phlebia radiata during Solid-State Fermentation of Wheat Straw , 1995, Applied and environmental microbiology.

[9]  C. Thurston.  The structure and function of fungal laccases , 1994 .

[10]  N. V. Van Alfen,et al.  Extra- and Intracellular Laccases of the Chestnut Blight Fungus, Cryphonectria parasitica , 1993, Applied and environmental microbiology.

[11]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[12]  R. Malkin,et al.  Spectroscopic differentiation of the electron-accepting sites in fungal laccase. Association of a near ultraviolet band with a two electron-accepting unit. , 1969, European journal of biochemistry.

[13]  T. Haltia,et al.  A novel combination of prosthetic groups in a fungal laccase; PQQ and two copper atoms , 1990, FEBS letters.

[14]  R. Huber,et al.  The blue oxidases, ascorbate oxidase, laccase and ceruloplasmin. Modelling and structural relationships. , 1990, European journal of biochemistry.

[15]  C. Thurston,et al.  Identification of two laccase genes in the cultivated mushroom Agaricus bisporus. , 1993, Journal of general microbiology.

[16]  L. Golovleva,et al.  Production of ligninolytic enzymes of the white rot fungus Panus tigrinus , 1994 .

[17]  A. Tsukamoto,et al.  Cloning, sequence analysis, and expression of ligninolytic phenoloxidase genes of the white-rot basidiomycete Coriolus hirsutus. , 1990, The Journal of biological chemistry.

[18]  T. Kirk,et al.  Laccase component of the Ceriporiopsis subvermispora lignin-degrading system , 1995, Applied and environmental microbiology.

[19]  W. Kooistra,et al.  FORMATION OF AN EXTRACELLULAR LACCASE BY A SCHIZOPHYLLUM-COMMUNE DIKARYON , 1986 .

[20]  J. Knowles,et al.  Isolation and structural analysis of the laccase gene from the lignin-degrading fungus Phlebia radiata. , 1991, Journal of general microbiology.

[21]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.