Serine substitution for cysteine residues in levansucrase selectively abolishes levan forming activity
暂无分享,去创建一个
[1] D. Eveleigh,et al. Identification of saccharolytic enzymes ofZymomonas mobilis CP4 , 1991, Biotechnology Letters.
[2] P. Gunasekaran,et al. Elevated temperature and chemical modification selectively abolishes levan forming activity of levansucrase of Zymomonas mobilis , 1999, Biotechnology Letters.
[3] P. Gunasekaran,et al. The sacB and sacC genes encoding levansucrase and sucrase form a gene cluster in Zymomonas mobilis , 1995, Biotechnology Letters.
[4] K. Okamoto,et al. Identification of functionally important amino acid residues in Zymomonas mobilis levansucrase. , 2002, Journal of biochemistry.
[5] P. Gunasekaran,et al. Polymerase and hydrolase activities of Zymomonas mobilis levansucrase separately modulated by in vitro mutagenesis and elevated temperature , 2001 .
[6] P. Gunasekaran,et al. A simple method for purification of thermostable levansucrase of Zymomonas mobilis from a recombinant Escherichia coli , 1998 .
[7] P. Gunasekaran,et al. Fermentation characteristics of levansucrase mutants of Zymomonas mobilis , 1993 .
[8] R. Chambert,et al. Polymerase and hydrolase activities of Bacillus subtilis levansucrase can be separately modulated by site-directed mutagenesis. , 1991, The Biochemical journal.
[9] F. Studier,et al. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. , 1986, Journal of molecular biology.
[10] L. Viikari. Formation of levan and sorbitol from sucrose by Zymomonas mobilis , 1984, Applied Microbiology and Biotechnology.
[11] M. M. Bradford. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.