Micellar electrokinetic capillary chromatography for the separation of cefalexin and its related substances

Micellar electrokinetic capillary chromatography (MEKC) was examined for analysis of cefalexin and its related substances. Good selectivity was obtained with two different buffer solutions: a sodium acetate buffer (50 mM, pH 5.25) containing sodium dodecyl sulfate (50 mM SDS) or sodium phosphate buffer (40 mM, pH 7.0) containing 100 mM SDS. Both methods permit cefalexin to be completely separated from its ten related substances within 20 min. The robustness of the method, using pH 5.25 acetate buffer, was examined by means of a full‐fraction factorial design to test the influence of buffer pH, concentration of SDS and buffer concentration. The parameters for validation such as linearity, precision, limit of detection and limit of quantitation are also reported. The results show that method 1 is suitable for the analysis of cefalexin.

[1]  Lutgarde M. C. Buydens,et al.  RES, AN EXPERT SYSTEM FOR THE SET-UP AND INTERPRETATION OF A RUGGEDNESS TEST IN HPLC METHOD VALIDATION .1. THE RUGGEDNESS TEST IN HPLC METHOD VALIDATION , 1991 .

[2]  M. Rey,et al.  Determination of pharmaceuticals by multi-phase chromatography : combined reversed phase and ion exchange in one column , 1990 .

[3]  C. Sciacchitano,et al.  Identification and separation of five cephalosporins by micellar electrokinetic capillary chromatography. , 1994, Journal of chromatography. B, Biomedical applications.

[4]  Shou‐Mei Wu,et al.  Micellar Electrokinetic Chromatography of Cephalosporin Antibiotics , 1996 .

[5]  R. Lantz,et al.  High-performance liquid chromatographic determination of loracarbef, a potential metabolite, cefaclor and cephalexin in human plasma, serum and urine. , 1991, Journal of chromatography.

[6]  H. Fabre,et al.  Evaluation of capillary zone electrophoresis and micellar electrokinetic capillary chromatography with direct injection of plasma for the determination of cefotaxime and its metabolite. , 1997, Analytical chemistry.

[7]  H. Fabre,et al.  Capillary Electrophoresis as an Alternative Method for the Determination of Cefotaxime , 1995 .

[8]  R. Neubert,et al.  Application of capillary zone electrophoresis in cephalosporin analysis. , 1997, Journal of chromatography. B, Biomedical sciences and applications.

[9]  E. Roets,et al.  Synthesis of Potential Impurities of Cefalexin and Cefradine , 1994 .

[10]  J. Haginaka,et al.  Direct high-speed liquid chromatographic determination of cephalexin in urine. , 1978, Journal of chromatography.

[11]  M. Rouan,et al.  Systematic approach to the determination of cephalosporins in biological fluids by reversed-phase liquid chromatography. , 1983, Journal of chromatography.

[12]  E. Roets,et al.  Quantitative analysis of cefalexin by liquid chromatography on poly(styrene-divinylbenzene) , 1992 .

[13]  H. Fabre,et al.  Cross validation of capillary electrophoresis and high-performance liquid chromatography for cefotaxime and related impurities , 1996 .

[14]  P. Emaldi,et al.  Validation of a capillary electrophoresis method for the determination of cephradine and its related impurities , 1995 .