Preparation of artificial antigen and polyclonal antibodies against deoxynivalenol
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Deoxnivalenol hapten was synthesized successfully followed by studying of the two kinds of hapten synthesis method. The immunogen was prepared following a scheme that employs active ester method for the conjugation of the haptens to BSA(bovine serum albumin), with which polyclonal antibodies against deoxynivalenol were raised in rabbits after immunization. The antiserum titer was 1∶32 000, and the antiserum was purified by proteinA-sepharose 4B immunity affinity chromatography. The antibody specificity was checked on the basis of the 50% inhibition levels, relative cross-reactivities with DON, 3-AC-DON, 15-AC-DON, T-2, OTA and ZEN were found to be 100%, 500%, 2.50%, 0.10%, 0.01%, 0.01%.