Lateral transport of a lipid probe and labeled proteins on a cell membrane.

Diffusion coefficients (D) of a lipid probe and labeled proteins on L-6 myoblast membranes have been measured giving D(protein) approximately 2 X 10(-10) square centimeter per second and D (lipid probe) approximately 9 X 10(-9) square centimeter per second. Some of the membrane proteins are immobile, but the lipid probe diffuses freely over macroscopic distances. Cytochalasin B slows protein but not lipid probe diffusion.

[1]  Mu-ming Poo,et al.  Lateral diffusion of rhodopsin in the photoreceptor membrane , 1974, Nature.

[2]  W. Webb,et al.  Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[3]  M. Bretscher Directed lipid flow in cell membranes , 1976, Nature.

[4]  M. Edidin,et al.  Lateral diffusion of concanavalin A receptors in the plasma membrane of mouse fibroblasts. , 1976, Biochimica et biophysica acta.

[5]  M Edidin,et al.  Measurement of membrane protein lateral diffusion in single cells. , 2003, Science.

[6]  K. Jacobson,et al.  Measurement of the translational mobility of concanavalin A in glycerol-saline solutions and on the cell surface by fluorescence recovery after photobleaching. , 1976, Biochimica et biophysica acta.

[7]  G M Edelman,et al.  Surface modulation in cell recognition and cell growth. , 1976, Science.

[8]  M. Bretscher,et al.  Mammalian plasma membranes , 1975, Nature.

[9]  W. Webb,et al.  Concentration correlation spectroscopy: a new biophysical probe based on occupation number fluctuations. , 1975, Annual review of biophysics and bioengineering.

[10]  M. Edidin,et al.  FLUIDITY OF THE SURFACE OF CULTURED MUSCLE FIBERS , 1973, The Journal of cell biology.

[11]  W. Webb,et al.  Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. , 1976, Biophysical journal.

[12]  P. Fahey,et al.  Lateral diffusion in planar lipid bilayers. , 1977, Science.

[13]  M Edidin,et al.  Rotational and translational diffusion in membranes. , 1974, Annual review of biophysics and bioengineering.

[14]  W. Webb,et al.  Dynamics of fluorescence marker concentration as a probe of mobility. , 1976, Biophysical journal.

[15]  G M Edelman,et al.  Restriction of the mobility of lymphocyte immunoglobulin receptors by concanavalin A. , 1972, Proceedings of the National Academy of Sciences of the United States of America.