Haemoglobins of the foetal and adult rat: sites of synthesis and the effects of erythropoietin.

Early studies on rat haemoglobins indicated the existence of several components (Gratzer & Allison, 1960). Seven components were found in the blood of adult random-bred Wistar rats by CM-cellulose chromatography; their rates of synthesis appeared unaltered during erythroid cell maturation (Brada & Tobiska, 1964). A 5-component electrophoretic pattern found in some randombred Wistar rats was considered to be a combination of two 4-component patterns observed in two inbred strains (Marinkovic, Martinovic & Kanazir, 1967). A 4-component electrophoretic pattern was also reported by Cole, Hunter & Paul (1968). During gestation the number of components resolved in this buffer system increased from 1 at 13 days to 4 at 15 days; the pattern appeared identical to that of the adult from 18 days onward. Erythropoietin stimulates stem cells to mature to erythrocytes (Filmanowicz & Gurney, 1961; Orlic, Gordon & Rhodin, 1965; Nakao, Miura & Takaku, 1966).

[1]  R. Cole,et al.  Hormonal Regulation of Pre‐natal Haemoglobin Synthesis by Erythropoietin , 1968, British journal of haematology.

[2]  A. de la Chapelle,et al.  Erythroid cell-development in fetal mice: synthetic capacity for different proteins. , 1968, Journal of molecular biology.

[3]  D. Kanazir,et al.  Electrophoretic Haemoglobin Patterns in One Random Bred and Two Inbred Strains of Laboratory Rats , 1967, Nature.

[4]  R. Cole,et al.  The effects of erythropoietin on haem synthesis in mouse yolk sac and cultured foetal liver cells. , 1966, Journal of embryology and experimental morphology.

[5]  A. S. Gordon,et al.  An ultrastructural study of erythropoietin-induced red cell formation in mouse spleen. , 1965, Journal of ultrastructure research.

[6]  A. Erslev.  THE ROLE OF ERYTHROPOIETIN IN THE CONTROL OF RED CELL PRODUCTION , 1964, Medicine.

[7]  M. Craig,et al.  A DEVELOPMENTAL CHANGE IN HEMOGLOBINS CORRELATED WITH AN EMBRYONIC RED CELL POPULATION IN THE MOUSE. , 1964, Developmental biology.

[8]  J. Tobiska,et al.  PROPERTIES OF RAT HAEMOGLOBIN. , 1964, Life sciences.

[9]  M. Craig,et al.  Electrophoretic Patterns of Hemoglobin from Fetal Mice of Different Inbred Strains , 1963, Science.

[10]  B. O'brien.  Development of haemoglobin by de-embryonated chick blastoderms cultured in vitro and the effect of abnormal RNA upon its synthesis. , 1961, Journal of embryology and experimental morphology.

[11]  A. Allison,et al.  MULTIPLE HAEMOGLOBINS , 1960, Biological reviews of the Cambridge Philosophical Society.

[12]  M. D. Poulik.  Starch Gel Electrophoresis in a Discontinuous System of Buffers , 1957, Nature.

[13]  O. Smithies,et al.  Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. , 1955, The Biochemical journal.

[14]  C. Gurney,et al.  Studies on erythropoiesis. XVI. Response to a single dose of erythropoietin in polycythemic mouse. , 1961, The Journal of laboratory and clinical medicine.