Crystal structure analysis of a complete turn of B-DNA

DNA is probably the most discussed and least observed of all biological macromolecules. Although its role in biology is a central one, with many examples such as operators and restriction sites where specific base sequences have control functions or interact with specific enzymes, the structures that DNA can adopt have been based until now only on sequence-averaged fibre diffraction patterns. Recent improvements in triester synthesis methods have made possible the preparation of sufficient homogeneous DNA of predetermined sequence for crystallization and X-ray structure analysis. We report here the first single-crystal structure analysis of more than a complete turn of right-handed B-DNA, with the self-complementary dodecamer sequence d(CpGpCpGpApApTpTpCpGpCpG) or CGCGAATTCGCG.

[1]  Michael Levitt,et al.  Refinement of Large Structures by Simultaneous Minimization of Energy and R Factor , 1978 .

[2]  M Levitt,et al.  How many base-pairs per turn does DNA have in solution and in chromatin? Some theoretical calculations. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[3]  J. Schellman,et al.  Flexibility of DNA , 1974, Biopolymers.

[4]  N. Seeman,et al.  Sequence-specific Recognition of Double Helical Nucleic Acids by Proteins (base Pairs/hydrogen Bonding/recognition Fidelity/ion Binding) , 2022 .

[5]  L. H. Wright,et al.  Variability in the tertiary structure of alpha-chymotrypsin at 2.8-A resolution. , 1973, Biochemistry.

[6]  E. O'brien.  Crystal structures of two complexes containing guanine and cytosine derivatives. , 1967, Acta crystallographica.

[7]  Jacques H. van Boom,et al.  Molecular structure of a left-handed double helical DNA fragment at atomic resolution , 1979, Nature.

[8]  D. V. Hoff,et al.  Cancer chemotherapy handbook , 1980 .

[9]  R. Dickerson,et al.  High-salt d(CpGpCpG), a left-handed Z′ DNA double helix , 1980, Nature.

[10]  G. Felsenfeld,et al.  A simple model of DNA superhelices in solution. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[11]  J. Wang,et al.  Helical repeat of DNA in solution. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[12]  D M Crothers,et al.  Transient electric dichroism of rod-like DNA molecules. , 1978, Proceedings of the National Academy of Sciences of the United States of America.