Electrically isolated buried electrode biosensor for detecting folic acid concentration

The current study sought to detect and quantify different folic acid concentrations in Phosphate Buffer Saline (PBS) by employing Electrical Impedance Spectroscopy (EIS).This technique provides a simple, rapid, precise and cost-effective platform for folic acid monitoring. An on-wafer platform has been developed to perform the necessary electrical measurements. Variation of electrical parameters such as impedance, capacitance and conductance for six different concentrations ranging from 4 mg/dl to 6 mg/dl are analyzed. The capacitance and conductance are observed to increase whereas impedance has been found to decrease with increasing folic acid content in saline. FT-MIR spectrum shows distinct confirmatory peaks relevant to the folic acid constituents in the IR range 4000 cm-1 to 400 cm-1.

[1]  S. Chattopadhyay,et al.  Low Frequency Impedimetric Cell Counting: Analytical Modeling and Measurements , 2020 .

[2]  K. Acharya,et al.  FT-MIR supported Electrical Impedance Spectroscopy based study of sugar adulterated honeys from different floral origin. , 2017, Talanta.

[3]  S. Chattopadhyay,et al.  On-chip detection and quantification of soap as an adulterant in milk employing electrical impedance spectroscopy , 2018, International Symposium on Devices, Circuits and Systems.

[4]  S. Chattopadhyay,et al.  Analytical modelling of electrical impedance based adulterant sensor for aqueous sucrose solutions , 2017 .

[5]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[6]  Tatsuo Togawa,et al.  Noninvasive Measurement of Hematocrit by Electrical Admittance Plethysmography Technique , 1980, IEEE Transactions on Biomedical Engineering.

[7]  K. Acharya,et al.  Impedimetric Approach for Estimating the Presence of Metanil Yellow in Turmeric Powder from Tunable Capacitance Measurement , 2019, Food Analytical Methods.

[8]  Parikha Mehrotra.  Biosensors and their applications - A review. , 2016, Journal of oral biology and craniofacial research.

[9]  Joho Yun,et al.  Ex vivo identification of thyroid cancer tissue using electrical impedance spectroscopy on a needle , 2018 .

[10]  Suryasnata Tripathy,et al.  A comprehensive approach for milk adulteration detection using inherent bio-physical properties as 'Universal Markers': Towards a miniaturized adulteration detection platform. , 2017, Food chemistry.

[11]  Josep Samitier,et al.  Advances in the production, immobilization, and electrical characterization of olfactory receptors for olfactory nanobiosensor development , 2006 .

[13]  R Bragós,et al.  In Vivo and In Situ Ischemic Tissue Characterization Using Electrical Impedance Spectroscopy a , 1999, Annals of the New York Academy of Sciences.

[14]  Fernando Seoane,et al.  An analog front-end enables electrical impedance spectroscopy system on-chip for biomedical applications , 2008, Physiological measurement.

[15]  A Rapid Chemiluminescence Assay for Measurement of Folate in Small Volumes of Breast Milk , 2019, Molecules.

[16]  G. S. Wilson,et al.  Electrochemical Biosensors: Recommended Definitions and Classification , 1999, Biosensors & bioelectronics.

[17]  Paul Geladi,et al.  Non‐invasive and microinvasive electrical impedance spectra of skin cancer – a comparison between two techniques , 2005, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[18]  Yu-Fai Lam,et al.  Self Association of Folic Acid in Aqueous Solution by Proton Magnetic Resonance , 1972 .

[19]  S. Chattopadhyay,et al.  Quantitative estimation of soda ash as an adulterant in aqueous sucrose solution by employing electrical impedance and capacitance spectroscopy , 2019 .

[20]  M. Arvand,et al.  A simple and efficient electrochemical sensor for folic acid determination in human blood plasma based on gold nanoparticles-modified carbon paste electrode. , 2013, Materials science & engineering. C, Materials for biological applications.

[21]  Uwe Schnakenberg,et al.  Electrical Impedance Spectroscopy of Single Cells in Hydrodynamic Traps , 2017 .

[22]  Subhadip Chakraborty,et al.  Investigating the quasi-oscillatory behavior of electrical parameters with the concentration of D-glucose in aqueous solution , 2015 .

[23]  Zhonghu Bai,et al.  Development of a Sensitive Chemiluminescence Immunoassay for the Quantification of Folic Acid in Human Serum , 2019, Journal of analytical methods in chemistry.

[24]  S. Chattopadhyay,et al.  Study of Optical and Electrical Characteristics of chemically extracted Lotus and Taro Bio-Wax for Hydrophobic Surface Engineering , 2019, 2019 International Conference on Opto-Electronics and Applied Optics (Optronix).

[25]  S. Chattopadhyay,et al.  Bio-dielectric variation as a signature of shape alteration and lysis of human erythrocytes: An on-chip analysis , 2018, 2018 International Symposium on Devices, Circuits and Systems (ISDCS).

[26]  Tsuyoshi Murata,et al.  {m , 1934, ACML.

[27]  Brigitte Maier.  Handbook Of Modern Sensors Physics Designs And Applications , 2016 .

[28]  J Jossinet,et al.  A Review of Parameters for the Bioelectrical Characterization of Breast Tissue , 1999, Annals of the New York Academy of Sciences.