Influence of extraction solvent system on the extractability of lipid components from the biomass of Nannochloropsis gaditana

Microalgae oils are considered to be promising alternative sources of omega-3 LC-PUFA. The aim of this work was therefore to evaluate different solvent (mixtures), currently accepted for use in the food industry, for the extraction of lipids from Nannochloropsis gaditana, an omega-3 LC-PUFA-rich microalga. Importantly, not only the total lipid yield but also the lipid class, eicosapentaenoic acid, carotenoid, and sterol yield were investigated. It was shown that the highest yield for each of the components was obtained with dichloromethane/ethanol (1:1). All extracts except the one obtained with dichloromethane/ethanol (1:1) were enriched in neutral lipids and depleted in polar lipids, when compared to the total lipid extract (chloroform/methanol 1:1). Hexane/isopropanol (3:2) seems to be the second best option: it has the advantage of performing better at criteria such as toxicity, but has the disadvantage that almost half of the interesting oil cannot be recovered.

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