Antimyeloperoxidase antibodies induce neutrophil adherence to cultured human endothelial cells.

Antimyeloperoxidase autoantibodies are found in the sera of some patients with glomerulonephritis and systemic vasculitis. Previously, we demonstrated that they were able to stimulate neutrophils to damage cultured human endothelial cells. We now report that antimyeloperoxidase antibodies are able to stimulate neutrophils to adhere to cultured human endothelial cells. Immunoglobulin G purified from myeloperoxidase-antineutrophil cytoplasmic autoantibody positive sera increased adherence to 331 +/- 60% of unstimulated controls. In a similar manner, rabbit antimyeloperoxidase enhanced neutrophil adherence. Stimulating the endothelial cells with 10 micrograms/mL endotoxin enhanced antimyeloperoxidase stimulated adherence. In the presence of a CD18 blocking antibody (MoAb 60.3), antimyeloperoxidase-stimulated adherence was significantly decreased. These results add further understanding to the antimyeloperoxidase-stimulated neutrophil-endothelial cell interaction and further support the hypothesis that antimyeloperoxidase autoantibodies are of pathogenic import in glomerulonephritis and vasculitis.

[1]  M. Keogan,et al.  Anti-neutrophil cytoplasm antibodies (ANCA) increase neutrophil adhesion to cultured human endothelium. , 1993, Advances in experimental medicine and biology.

[2]  J. Pearson,et al.  Autoantibodies developing to myeloperoxidase and proteinase 3 in systemic vasculitis stimulate neutrophil cytotoxicity toward cultured endothelial cells. , 1992, The American journal of pathology.

[3]  R. Falk,et al.  Anti-myeloperoxidase antibodies stimulate neutrophils to damage human endothelial cells. , 1992, Kidney international.

[4]  R. Falk,et al.  The pathogenic role of antineutrophil cytoplasmic autoantibodies. , 1991, American journal of kidney diseases : the official journal of the National Kidney Foundation.

[5]  R. Coppo,et al.  Classic and perinuclear anti-neutrophil cytoplasm antibodies and antimyeloperoxidase antibodies in rapidly progressive glomerulonephritis. , 1991, American journal of nephrology.

[6]  T. Carey,et al.  Clinical course of anti-neutrophil cytoplasmic autoantibody-associated glomerulonephritis and systemic vasculitis. The Glomerular Disease Collaborative Network. , 1990, Annals of internal medicine.

[7]  R J Falk,et al.  Anti-neutrophil cytoplasmic autoantibodies induce neutrophils to degranulate and produce oxygen radicals in vitro. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[8]  R. Falk.  ANCA-associated renal disease. , 1990, Kidney international.

[9]  A. Barrett,et al.  Effect of methotrexate on relapse after bone-marrow transplantation for acute lymphoblastic leukaemia , 1989 .

[10]  R. Falk,et al.  Anti-neutrophil cytoplasmic autoantibodies with specificity for myeloperoxidase in patients with systemic vasculitis and idiopathic necrotizing and crescentic glomerulonephritis. , 1988, The New England journal of medicine.

[11]  G. Zimmerman,et al.  Neutrophil adherence to human endothelium in vitro occurs by CDw18 (Mo1, MAC-1/LFA-1/GP 150,95) glycoprotein-dependent and -independent mechanisms. , 1988, The Journal of clinical investigation.

[12]  H. Ochs,et al.  An endothelial cell surface factor(s) induced in vitro by lipopolysaccharide, interleukin 1, and tumor necrosis factor-alpha increases neutrophil adherence by a CDw18-dependent mechanism. , 1986, Journal of immunology.

[13]  R. Schleimer,et al.  Cultured human vascular endothelial cells acquire adhesiveness for neutrophils after stimulation with interleukin 1, endotoxin, and tumor-promoting phorbol diesters. , 1986, Journal of immunology.

[14]  T. H. The,et al.  AUTOANTIBODIES AGAINST NEUTROPHILS AND MONOCYTES: TOOL FOR DIAGNOSIS AND MARKER OF DISEASE ACTIVITY IN WEGENER'S GRANULOMATOSIS , 1985, The Lancet.

[15]  I. Goldstein,et al.  Adherence of human polymorphonuclear leukocytes to endothelial monolayers: effects of temperature, divalent cations, and chemotactic factors on the strength of adherence measured with a new centrifugation assay , 1985 .

[16]  P. Beatty,et al.  ABSENCE OF MONOCLONAL-ANTIBODY-DEFINED PROTEIN COMPLEX IN BOY WITH ABNORMAL LEUCOCYTE FUNCTION , 1984, The Lancet.