Patients with Alzheimer’s Disease

We developed a novel quantitative assay to test the hypothesis that defects in DNA cytosine methylation might be responsible for the brain chromatin abnormalities and transcriptional alterations observed in patients with Alzheimer’s disease (AD). We found no significant difference in percent methylation of CCGG sites from brain DNA of 44 patients with AD compared with 20 normal subjects. These results, however, would not exclude genomic redistribution of methylcytosine in AD, or disturbed methylation of a limited population of critical brain-specific genes.

[1]  Alzheimer’s Association.  2018 Alzheimer's disease facts and figures , 2018, Alzheimer's & Dementia.

[2]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[3]  M. Zeviani,et al.  Tissue distribution and transmission of mitochondrial DNA deletions in mitochondrial myopathies , 1990, Annals of neurology.

[4]  A. Clark,et al.  Altered expression of genes for amyloid and cytoskeletal proteins in alzheimer cortex , 1989, Annals of neurology.

[5]  P. C. Rath,et al.  Methylation of repetitive DNA sequences in the brain during aging of the rat , 1989, FEBS letters.

[6]  J. Sutcliffe,et al.  Primate brain-specific cytoplasmic transcript of the Alu repeat family , 1987, Molecular and cellular biology.

[7]  V. Wilson,et al.  Genomic 5-methyldeoxycytidine decreases with age. , 1987, The Journal of biological chemistry.

[8]  P. N. Lewis,et al.  Characterization of Messenger RNA from the Cerebral Cortex of Control and Alzheimer‐Afflicted Brain , 1986, Journal of neurochemistry.

[9]  H. Cedar,et al.  Effect of regional DNA methylation on gene expression. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[10]  P. N. Lewis,et al.  Chromatin structure in dementia , 1984, Annals of neurology.

[11]  D. S. Gross,et al.  5-methylcytosine is localized in nucleosomes that contain histone H1. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[12]  J. Hurst,et al.  DNA methylation and the regulation of globin gene expression , 1983, Cell.

[13]  V. L. Wilson,et al.  DNA methylation decreases in aging but not in immortal cells. , 1983, Science.

[14]  M. Kastan,et al.  Methylation of deoxycytidine incorporated by excision-repair synthesis of DNA , 1982, Cell.

[15]  S. Goldstein,et al.  Interclonal variation in methylation patterns for expressed and non-expressed genes. , 1982, Nucleic acids research.

[16]  P. N. Lewis,et al.  Changes in Chromatin Structure Associated with Alzheimer's Disease , 1981, Journal of neurochemistry.

[17]  T. Maniatis,et al.  Tissue-specific DNA methylation in a cluster of rabbit beta-like globin genes. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[18]  A. Bird,et al.  Use of restriction enzymes to detect potential gene sequences in mammalian DNA , 1987, Nature.

[19]  V. Klimenko,et al.  The 5-methylcytosine in DNA of rats. Tissue and age specificity and the changes induced by hydrocortisone and other agents. , 1973, Gerontologia.