Nucleic-acid immobilization, recognition and detection at chronopotentiometric DNA chips.

Wide-scale DNA testing requires the development of fast, small, easy-to-use biosensing devices. Various synthetic oligonucleotides and DNA have thus been immobilized onto microfabricated thick-film carbon transducers for performing several new nucleic-acid assay protocols. These include hybridization detection of nucleic acid sequences, determination of small molecules (drugs, pollutants) based on their collection into the dsDNA layer or via monitoring their effect upon the intrinsic DNA oxidation signal, and direct adsorptive stripping measurements of ultratrace levels of nucleic acids. Transduction of these DNA recognition processes is accomplished by a new highly-sensitive constant-current stripping chronopotentiometric operation. Comparison to traditional electrodes indicates that the biosensing performance is not compromised by the use of mass-producible disposable transducers. Such thick-film DNA biosensors have been coupled to a compact, user-friendly, hand-held analyzer. Applicability for the detection of sequences from M. tuberculosis and HIV-1 DNAs is illustrated. Such activity in the author's laboratory, aimed at developing DNA-coated screen-printed electrodes, is reviewed.

[1]  A. M. Brett,et al.  Electrochemical determination of carboplatin in serum using a DNA‐modified glassy carbon electrode , 1996 .

[2]  L. McGown,et al.  The nucleic acid ligand. A new tool for molecular recognition. , 1995, Analytical chemistry.

[3]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[4]  Gustavo Rivas,et al.  Stripping potentiometric transduction of DNA hybridization processes , 1996 .

[5]  Joseph Wang,et al.  Adsorptive stripping potentiometry of DNA at electrochemically pretreated carbon paste electrodes , 1996 .

[6]  R. Mason,et al.  ESR spectroscopy of flow-oriented cation radicals of phenothiazine derivatives and phenoxathiin intercalated in DNA. , 1991, Chemico-biological interactions.

[7]  L J Kricka,et al.  PCR in a silicon microstructure. , 1994, Clinical chemistry.

[8]  J Wang,et al.  DNA electrochemical biosensor for the detection of short DNA sequences related to the human immunodeficiency virus. , 1996, Analytical chemistry.

[9]  P. C. Pandey,et al.  Detection of aromatic compounds based on DNA intercalation using an evanescent wave biosensor , 1995 .

[10]  E. Paleček,et al.  From polarography of DNA to microanalysis with nucleic acid-modified electrodes , 1996 .

[11]  Susan R. Mikkelsen,et al.  Covalent immobilization of DNA onto glassy carbon electrodes , 1992 .

[12]  G. Rivas,et al.  Accumulation and trace measurements of phenothiazine drugs at DNA-modified electrodes , 1996 .

[13]  Joseph Wang,et al.  Decentralized electrochemical monitoring of trace metals: from disposable strips to remote electrodes. Plenary lecture , 1994 .

[14]  X. Cai,et al.  DNA biosensor for the detection of hydrazines. , 1996, Analytical chemistry.

[15]  Peter E. Nielsen,et al.  Peptide Nucleic Acid Probes for Sequence-Specific DNA Biosensors , 1996 .

[16]  Liang-Chy Chien,et al.  Nucleic Acid Hybridization Detected by Piezoelectric Resonance , 1988 .

[17]  D. Crothers,et al.  Studies on interaction of anthracycline antibiotics and deoxyribonucleic acid: equilibrium binding studies on interaction of daunomycin with deoxyribonucleic acid. , 1982, Biochemistry.