Preparation and optical scattering characterization of gold nanorods and their application to a dot-immunogold assay.

We describe optical monitoring of the synthesis of gold nanorods (NRs) based on seed-mediated growth in the presence of the soft surfactant template cetyltrimethyilammonium bromide. To separate NRs from spheres and surfactants we fractionated samples in the density gradient of glycerol. The optical properties of NRs were characterized by extinction and differential light-scattering spectra (at 90 degrees, 450-800 nm) and by the depolarization light-scattering ratio, I(vh)/I(vv), measured at 90 degrees with a helium-neon laser. Theoretical spectra and the I(vh)/I(vv) ratios were calculated by the T-matrix method as applied to randomly oriented NRs, which were modeled by right-circular cylinders with semispherical ends. The simulated data were fitted to experimental observations by use of particle length and width as adjustable parameters, which were close to the data yielded by transmission electron microscopy. The sensitivity of the long-wavelength resonance of NRs to the dielectric surroundings was examined both experimentally and theoretically by comparison of the extinction spectra of NRs in water and in a 25% glycerol solution. Finally, we discuss the application of NR-protein A conjugates to a dot-immunogold assay with the example of biospecific staining of human IgG molecules adsorbed onto small membrane spots.

[1]  Huimeng Wu,et al.  Oriented assembly of Au nanorods using biorecognition system. , 2005, Chemical communications.

[2]  Milton Kerker,et al.  The Scattering of Light and Other Electromagnetic Radiation ~Academic , 1969 .

[3]  S. Bruzzone,et al.  Some spectroscopic properties of gold nanorods according to a schematic quantum model founded on the dielectric behavior of the electron-gas confined in a box. I , 2003 .

[4]  Latha A. Gearheart,et al.  Anisotropic Chemical Reactivity of Gold Spheroids and Nanorods , 2002 .

[5]  Paul Mulvaney,et al.  Drastic reduction of plasmon damping in gold nanorods. , 2002 .

[6]  George C. Schatz,et al.  A nanoscale optical biosensor: The long range distance dependence of the localized surface plasmon resonance of noble metal nanoparticles , 2004 .

[7]  H. V. Hulst.  Light Scattering by Small Particles , 1957 .

[8]  D. Pang,et al.  Resonance light scattering spectroscopy study of interaction between gold colloid and thiamazole and its analytical application. , 2004, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[9]  A. V. Alekseeva,et al.  Can the light scattering depolarization ratio of small particles be greater than 1/3? , 2005, The journal of physical chemistry. B.

[10]  A Paul Alivisatos,et al.  Gold nanorods as novel nonbleaching plasmon-based orientation sensors for polarized single-particle microscopy. , 2005, Nano letters.

[11]  Lin He,et al.  Particles for multiplexed analysis in solution: detection and identification of striped metallic particles using optical microscopy. , 2002, Analytical chemistry.

[12]  T. Klar,et al.  Biomolecular Recognition Based on Single Gold Nanoparticle Light Scattering , 2003 .

[13]  Adam D. McFarland,et al.  Single Silver Nanoparticles as Real-Time Optical Sensors with Zeptomole Sensitivity , 2003 .

[14]  M. Moeremans,et al.  The Preparation of Colloidal Gold Probes and Their Use as Marker in Electron Microscopy , 1986 .

[15]  George C. Schatz,et al.  Surface plasmon broadening for arbitrary shape nanoparticles: A geometrical probability approach , 2003 .

[16]  Mostafa A. El-Sayed,et al.  Preparation and Growth Mechanism of Gold Nanorods (NRs) Using Seed-Mediated Growth Method , 2003 .

[17]  Stephan Link,et al.  Optical properties and ultrafast dynamics of metallic nanocrystals. , 2003, Annual review of physical chemistry.

[18]  Nikolai G. Khlebtsov,et al.  Optical Properties and Biomedical Applications of Nanostructures Based on Gold and Silver Bioconjugates , 2004 .

[19]  N. Jana,et al.  Preparation of Polystyrene- and Silica-Coated Gold Nanorods and Their Use as Templates for the Synthesis of Hollow Nanotubes , 2001 .

[20]  N. Khlebtsov,et al.  A new spectral resonance of metallic nanorods , 2004 .

[21]  V. G. Farafonov,et al.  Optical properties of spheroidal particles , 1993 .

[22]  R. V. Van Duyne,et al.  A nanoscale optical biosensor: sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles. , 2002, Journal of the American Chemical Society.

[23]  M. Natan,et al.  Seeding of Colloidal Au Nanoparticle Solutions. 2. Improved Control of Particle Size and Shape , 2000 .

[24]  J. Lakowicz,et al.  Metallic colloid wavelength-ratiometric scattering sensors. , 2003, Analytical chemistry.

[25]  Christine M. Micheel,et al.  Biological applications of colloidal nanocrystals , 2003 .

[26]  M. El-Sayed,et al.  The `lightning' gold nanorods: fluorescence enhancement of over a million compared to the gold metal , 2000 .

[27]  Nikolai G. Khlebtsov,et al.  Plasmon resonances of silver and gold nanorods , 2004, Saratov Fall Meeting.

[28]  N. G. Khlebtsov,et al.  The effect of the size, shape, and structure of metal nanoparticles on the dependence of their optical properties on the refractive index of a disperse medium , 2005 .

[29]  J. Yguerabide,et al.  Light-scattering submicroscopic particles as highly fluorescent analogs and their use as tracer labels in clinical and biological applications. , 1998, Analytical biochemistry.

[30]  D. Astruc,et al.  Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. , 2004, Chemical reviews.

[31]  Differential Light Scattering Spectroscopy for Studying Biospecific Assembling of Gold Nanoparticles with Protein or Oligonucleotide Probes , 2002 .

[32]  Catherine J. Murphy,et al.  Seed‐Mediated Growth Approach for Shape‐Controlled Synthesis of Spheroidal and Rod‐like Gold Nanoparticles Using a Surfactant Template , 2001 .

[33]  Nikolai G. Khlebtsov,et al.  Differential light-scattering spectroscopy: a new approach to studying of colloidal gold nanosensors , 2004 .

[34]  Nikolai G. Khlebtsov,et al.  Spectral Extinction of Colloidal Gold and Its Biospecific Conjugates , 1996 .

[35]  Nikolai G. Khlebtsov,et al.  Light-scattering spectra of colloidal gold aggregates: experimental measurements and theoretical simulations , 2001, Saratov Fall Meeting.

[36]  J. Yguerabide,et al.  Light-scattering submicroscopic particles as highly fluorescent analogs and their use as tracer labels in clinical and biological applications. , 1998, Analytical biochemistry.

[37]  Catherine J. Murphy,et al.  Wet Chemical Synthesis of High Aspect Ratio Cylindrical Gold Nanorods , 2001 .

[38]  J. Yi,et al.  Aspect ratio controlled synthesis of gold nanorods , 2003 .

[39]  Z. Kam,et al.  Absorption and Scattering of Light by Small Particles , 1998 .

[40]  Aristides A. G. Requicha,et al.  Imaging and manipulation of gold nanorods with an atomic force microscope , 2002 .

[41]  Nikolai G. Khlebtsov,et al.  Optical properties of colloidal gold and its conjugates with biospecific macromolecules , 1995 .

[42]  C. Schönenberger,et al.  Colloidal Dispersions of Gold Rods: Synthesis and Optical Properties , 2000 .

[43]  Zhiliang Jiang,et al.  Resonance scattering spectroscopy of gold nanoparticle , 2001 .

[44]  N. Khlebtsov,et al.  Orientational averaging of light-scattering observables in the J-matrix approach. , 1992, Applied optics.

[45]  Wei Chen,et al.  Sonochemical Processes and Formation of Gold Nanoparticles within Pores of Mesoporous Silica. , 2001, Journal of colloid and interface science.

[46]  Andrew A. Lacis,et al.  Scattering, Absorption, and Emission of Light by Small Particles , 2002 .

[47]  G. Xu,et al.  Preparation and assembly of colloidal gold nanoparticles in CTAB-stabilized reverse microemulsion , 2003 .

[48]  R. Gans,et al.  Über die Form ultramikroskopischer Goldteilchen , 1912 .

[49]  M. El-Sayed,et al.  Simulation of the Optical Absorption Spectra of Gold Nanorods as a Function of Their Aspect Ratio and the Effect of the Medium Dielectric Constant , 1999 .

[50]  Gerald J. Meyer,et al.  Biological applications of high aspect ratio nanoparticles , 2004 .

[51]  Boris N. Khlebtsov,et al.  Measurement of mean size and evaluation of polydispersity of gold nanoparticles from spectra of optical absorption and scattering , 2004 .

[52]  E. Coronado,et al.  The Optical Properties of Metal Nanoparticles: The Influence of Size, Shape, and Dielectric Environment , 2003 .

[53]  David A. Schultz,et al.  Plasmon resonant particles for biological detection. , 2003, Current opinion in biotechnology.

[54]  David R. Smith,et al.  Local Refractive Index Dependence of Plasmon Resonance Spectra from Individual Nanoparticles , 2003 .

[55]  B. Nikoobakht,et al.  種結晶を媒介とした成長法を用いた金ナノロッド(NR)の調製と成長メカニズム , 2003 .

[56]  Jae Hee Song,et al.  Photochemical synthesis of gold nanorods. , 2002, Journal of the American Chemical Society.

[57]  V. A. Bogatyrev,et al.  COLLOIDAL GOLD IN SOLID-PHASE ASSAYS. A REVIEW , 1997 .

[58]  M. El-Sayed,et al.  Spectral Properties and Relaxation Dynamics of Surface Plasmon Electronic Oscillations in Gold and Silver Nanodots and Nanorods , 1999 .