Fluorometric Determination of Kynurenic Acid in Human Serum by High Performance Liquid Chromatography Coupled with Postcolumn Photochemical Reaction with Hydrogen Peroxide

A high performance liquid chromatographic method involving postcolumn photochemical reaction and fluorometric detection has been developed for the determination of kynurenic acid. Kynurenic acid was separated on a reversed phase column with a mobile phase containing hydrogen peroxide and irradiated by ultraviolet light to give fluorescence. The method allows the determination of kynurenic acid in human serum at concentrations as low as 0.05pmol/100μl.