Analysis and experiment of centrifugal force for microfluidic ELISA CD platform

This paper presents an analysis and experiment of centrifugal force for microfluidic Enzyme-Linked Immunosorbent Assay (ELISA) on a compact-disc (CD) platform. The ELISA CD was designed based on the centrifugal force as a driving force while capillary force acts as preventing barrier. The CD composed of 2 layers: the substrate layer is made of PMMA and the top layer is laminated with an Adhesive Sealing Films (ASF) or thermal seal. The PMMA substrate was fabricated by CNC micromachining. The ELISA CD consists of 7 reservoirs: waste, detection, serum, conjugate, washing solution, substrate and stopping solution. In the experiment, the reservoirs were filled with colour liquid and the fluid flow behaviour of the liquid in the CD was studied and monitored by a customized CD spin stand system equipped with visualization system. The experimental test results show an average of 9% error when compared with the theoretical calculation of burst frequency for all the reservoirs.

[1]  R. Zengerle,et al.  Visualization of flow patterning in high-speed centrifugal microfluidics , 2005 .

[2]  R. Zengerle,et al.  Single-step centrifugal hematocrit determination on a 10-$ processing device , 2007, Biomedical microdevices.

[3]  Eric D Salin,et al.  Rapid simultaneous determination of nitrate and nitrite on a centrifugal microfluidic device. , 2010, Talanta.

[4]  M. Bergeron.  Revolutionizing the practice of medicine through rapid (< 1h) DNA-based diagnostics. , 2008, Clinical and investigative medicine. Medecine clinique et experimentale.

[5]  Alain Glière,et al.  Modeling and fabrication of capillary stop valves for planar microfluidic systems , 2006 .

[6]  Weixiong Wang,et al.  Design and testing of a microfluidic biochip for cytokine enzyme-linked immunosorbent assay. , 2009, Biomicrofluidics.

[7]  Marc Madou,et al.  Pneumatic pumping in centrifugal microfluidic platforms , 2010 .

[8]  Marc Madou,et al.  Lab on a CD. , 2006, Annual review of biomedical engineering.

[9]  Eric D Salin,et al.  Automated liquid-solid extraction of pyrene from soil on centrifugal microfluidic devices. , 2010, Talanta.

[10]  Ángel Maquieira,et al.  Analytical prospect of compact disk technology in immunosensing , 2008, Analytical and bioanalytical chemistry.

[11]  A novel splitter design for microfluidic biochips using centrifugal driving forces , 2010 .

[12]  Bingcheng Lin,et al.  Rapid nanoliter DNA hybridization based on reciprocating flow on a compact disk microfluidic device. , 2009, Analytica chimica acta.

[13]  Gunnar Kylberg,et al.  Parallel nanoliter microfluidic analysis system. , 2007, Analytical chemistry.

[14]  M. Bergeron,et al.  Validation of a centrifugal microfluidic sample lysis and homogenization platform for nucleic acid extraction with clinical samples. , 2010, Lab on a chip.

[15]  Jim Zoval,et al.  Automated microfluidic compact disc (CD) cultivation system of Caenorhabditis elegans , 2007 .

[16]  Wen J. Li,et al.  Design and fabrication of centrifugal microfluidic disk for allergic response monitoring , 2009, 2009 14th OptoElectronics and Communications Conference.

[17]  Kuo-Sheng Ma,et al.  Dynamic automated DNA hybridization on a CD (compact disc) fluidic platform , 2006 .

[18]  Hansheng Pan,et al.  Reciprocating flow-based centrifugal microfluidics mixer. , 2009, The Review of scientific instruments.

[19]  Eric D. Salin,et al.  Pre-concentration of trace metals on centrifugal microfluidic discs with direct determination by laser ablation inductively coupled plasma mass spectrometry , 2009 .

[20]  Roland Zengerle,et al.  Rapid prototyping of microfluidic chips in COC , 2007 .

[21]  Roland Zengerle,et al.  Direct hemoglobin measurement on a centrifugal microfluidic platform for point-of-care diagnostics , 2006 .